Human REN ELISA Kit

Alternative name
Human angiotensinogenase ELISA Kit;Human HNFJ2 ELISA Kit;Human renin ELISA Kit;Human angiotensin-forming enzyme ELISA Kit;Human renin precursor, renal ELISA Kit;
Catalog
E033189
species
Human
GeneREN
SpecificityNatural and recombinant human Renin
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman REN ELISA Kit allows for the in vitro quantitative determination of REN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Renin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Renin has been precoated onto 96-well plates. Standards(NSO, L67-R406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Renin amount of sample captured in plate. Background: Renin, also known as an angiotensinogenase, is an enzyme that participates in the body's renin-angiotensin system(RAS). This gene is mapped to 1q32.1. Renin's primary function is to cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys. It also can catalyze the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. What's more, Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through beta-1 adrenoceptor activation.
Human Renin Protein information
Uniprot ID RENI_HUMAN
Uniprot AC P00797; Q6FI38; Q6T5C2;
UniGene Hs.3210;
GeneID 5972
KEGG hsa:5972;
Human Renin Protein SEQUENCE
SEQUENCE 406 AA; 45057 MW; 5AFDF8E973B21EDA CRC64;
MDGWRRMPRW GLLLLLWGSC TFGLPTDTTT FKRIFLKRMP SIRESLKERG
VDMARLGPEW SQPMKRLTLG NTTSSVILTN YMDTQYYGEI GIGTPPQTFK
VVFDTGSSNV WVPSSKCSRL YTACVYHKLF DASDSSSYKH NGTELTLRYS
TGTVSGFLSQ DIITVGGITV TQMFGEVTEM PALPFMLAEF DGVVGMGFIE
QAIGRVTPIF DNIISQGVLK EDVFSFYYNR DSENSQSLGG QIVLGGSDPQ
HYEGNFHYIN LIKTGVWQIQ MKGVSVGSST LLCEDGCLAL VDTGASYISG
STSSIEKLME ALGAKKRLFD YVVKCNEGPT LPDISFHLGG KEYTLTSADY
VFQESYSSKK LCTLAIHAMD IPPPTGPTWA LGATFIRKFY TEFDRRNNRI
GFALAR
UCSC uc001haq.3; human. [P00797-1];

Category

Human REN ELISA Kit

Human angiotensinogenase ELISA Kit;Human HNFJ2 ELISA Kit;Human renin ELISA Kit;Human angiotensin-forming enzyme ELISA Kit;Human renin precursor, renal ELISA Kit;