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Alternative name
Human Calvasculin ELISA Kit;Human Metastasin ELISA Kit;Human placental calcium-binding protein ELISA Kit;Human protein Mts1 ELISA Kit;Human S100 calcium-binding protein A4 ELISA Kit;Human CAPL ELISA Kit;Human MTS1 ELISA Kit;Human 18A2 ELISA Kit;Human 42A ELISA Kit;Human FSP1 ELISA Kit;Human P9KA ELISA Kit;Human PEL98 ELISA Kit;Human S100 calcium binding protein A4 ELISA Kit;Human protein S100-A4 ELISA Kit;Human S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) ELISA Kit;Human fibroblast-specific protein-1 ELISA Kit;Human leukemia multidrug resistance associated protein ELISA Kit;Human malignant transformation suppression 1 ELISA Kit;
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Catalog
E034094
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species
Human
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GeneS100A4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A4 (S100A4).
No significant cross-reactivity or interference between S100 Calcium Binding Protein A4 (S100A4) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 27pg/mL.
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Intended UseHuman S100A4 ELISA Kit allows for the in vitro quantitative determination of S100A4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A4 (S100A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A4 (S100A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A4 (S100A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A4 (S100A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Protein S100-A4 Protein information
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Uniprot ID
S10A4_HUMAN
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Uniprot AC
P26447; A8K7R8; D3DV46; Q6ICP8;
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UniGene
Hs.654444;
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GeneID
6275
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KEGG
hsa:6275;
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Human Protein S100-A4 Protein SEQUENCE
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SEQUENCE 101 AA; 11729 MW; 286D2B7B07EDB562 CRC64;
MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT
DEAAFQKLMS
NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK
K
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UCSC
uc001fby.4; human.;
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