Human SCTR ELISA Kit

Alternative name
Human SCT-R ELISA Kit;Human SR ELISA Kit;Human secretin receptor ELISA Kit;Human pancreatic secretin receptor ELISA Kit;
Catalog
E034464
species
Human
GeneSCTR
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman SCTR ELISA Kit allows for the in vitro quantitative determination of SCTR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: SCTR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SCTR. Standards or samples are then added to the microtiter plate wells and SCTR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SCTR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for SCTR are added to each well to "sandwich" the SCTR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SCTR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCTR concentration in each sample is interpolated from this standard curve.
Human Secretin receptor Protein information
Uniprot ID SCTR_HUMAN
Uniprot AC P47872; Q12961; Q13213; Q53T00;
UniGene Hs.42091;
GeneID 6344
KEGG hsa:6344;
Human Secretin receptor Protein SEQUENCE
SEQUENCE 440 AA; 50207 MW; E22CDD0EE7C0ACC1 CRC64;
MRPHLSPPLQ QLLLPVLLAC AAHSTGALPR LCDVLQVLWE EQDQCLQELS
REQTGDLGTE QPVPGCEGMW DNISCWPSSV PGRMVEVECP RFLRMLTSRN
GSLFRNCTQD GWSETFPRPN LACGVNVNDS SNEKRHSYLL KLKVMYTVGY
SSSLVMLLVA LGILCAFRRL HCTRNYIHMH LFVSFILRAL SNFIKDAVLF
SSDDVTYCDA HRAGCKLVMV LFQYCIMANY SWLLVEGLYL HTLLAISFFS
ERKYLQGFVA FGWGSPAIFV ALWAIARHFL EDVGCWDINA NASIWWIIRG
PVILSILINF ILFINILRIL MRKLRTQETR GNEVSHYKRL ARSTLLLIPL
FGIHYIVFAF SPEDAMEIQL FFELALGSFQ GLVVAVLYCF LNGEVQLEVQ
KKWQQWHLRE FPLHPVASFS NSTKASHLEQ SQGTCRTSII
UCSC uc002tma.4; human.;

Category

Human SCTR ELISA Kit

Human SCT-R ELISA Kit;Human SR ELISA Kit;Human secretin receptor ELISA Kit;Human pancreatic secretin receptor ELISA Kit;