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Human SEMA4A ELISA Kit

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  • Alternative name

    Human semaphorin-B ELISA Kit;Human sema B ELISA Kit;Human SEMAB ELISA Kit;Human SEMB ELISA Kit;Human CORD10 ELISA Kit;Human RP35 ELISA Kit;Human semaphorin 4A ELISA Kit;Human semaphorin-4A ELISA Kit;Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A ELISA Kit;
  • Catalog
    E034567
  • species
    Human
  • GeneSEMA4A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SEMA4A. No significant cross-reactivity or interference between human SEMA4A and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SEMA4A is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseHuman SEMA4A ELISA Kit allows for the in vitro quantitative determination of SEMA4A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Semaphorin-4A Protein information
  • Uniprot ID SEM4A_HUMAN
  • Uniprot AC Q9H3S1; B2RDH8; B3KR76; Q5TCI5; Q5TCJ6; Q8WUA9;
  • UniGene Hs.408846;
  • GeneID 64218
  • KEGG hsa:64218;
  • Human Semaphorin-4A Protein SEQUENCE

  • SEQUENCE 761 AA; 83574 MW; 5BCB889AA32A2BB3 CRC64;

    MALPALGLDP WSLLGLFLFQ LLQLLLPTTT AGGGGQGPMP RVRYYAGDER

    RALSFFHQKG LQDFDTLLLS GDGNTLYVGA REAILALDIQ DPGVPRLKNM

    IPWPASDRKK SECAFKKKSN ETQCFNFIRV LVSYNVTHLY TCGTFAFSPA

    CTFIELQDSY LLPISEDKVM EGKGQSPFDP AHKHTAVLVD GMLYSGTMNN

    FLGSEPILMR TLGSQPVLKT DNFLRWLHHD ASFVAAIPST QVVYFFFEET

    ASEFDFFERL HTSRVARVCK NDVGGEKLLQ KKWTTFLKAQ LLCTQPGQLP

    FNVIRHAVLL PADSPTAPHI YAVFTSQWQV GGTRSSAVCA FSLLDIERVF

    KGKYKELNKE TSRWTTYRGP ETNPRPGSCS VGPSSDKALT FMKDHFLMDE

    QVVGTPLLVK SGVEYTRLAV ETAQGLDGHS HLVMYLGTTT GSLHKAVVSG

    DSSAHLVEEI QLFPDPEPVR NLQLAPTQGA VFVGFSGGVW RVPRANCSVY

    ESCVDCVLAR DPHCAWDPES RTCCLLSAPN LNSWKQDMER GNPEWACASG

    PMSRSLRPQS RPQIIKEVLA VPNSILELPC PHLSALASYY WSHGPAAVPE

    ASSTVYNGSL LLIVQDGVGG LYQCWATENG FSYPVISYWV DSQDQTLALD

    PELAGIPREH VKVPLTRVSG GAALAAQQSY WPHFVTVTVL FALVLSGALI

    ILVASPLRAL RARGKVQGCE TLRPGEKAPL SREQHLQSPK ECRTSASDVD

    ADNNCLGTEV A

  • UCSC uc001fnl.4; human. [Q9H3S1-1];



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