Human SIRPG ELISA Kit

Alternative name
Human SIRP-gamma ELISA Kit;Human CD172 antigen-like family member B ELISA Kit;Human signal-regulatory protein beta-2 ELISA Kit;Human SIRP-B2 ELISA Kit;Human SIRP-beta-2 ELISA Kit;Human SIRPB2 ELISA Kit;Human CD172g ELISA Kit;Human SIRPgamma ELISA Kit;Human bA77C3.1 ELISA Kit;Human signal regulatory protein gamma ELISA Kit;Human signal-regulatory protein gamma ELISA Kit;Human CD172g antigen ELISA Kit;Human SIRP beta 2 ELISA Kit;
Catalog
E035248
species
Human
GeneSIRPG
SpecificityThis assay has high sensitivity and excellent specificity for detection of SIRPg. No significant cross-reactivity or interference between SIRPg and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of SIRPg is typically less than 0.067ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseHuman SIRPG ELISA Kit allows for the in vitro quantitative determination of SIRPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRPg. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SIRPg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SIRPg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SIRPg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Signal-regulatory protein gamma Protein information
Uniprot ID SIRPG_HUMAN
Uniprot AC Q9P1W8; B1AKP6; Q5D051; Q5JV25; Q5MKL4; Q8WWA5; Q9NQK8;
UniGene Hs.590883;
GeneID 55423
KEGG hsa:55423;
Human Signal-regulatory protein gamma Protein SEQUENCE
SEQUENCE 387 AA; 42498 MW; 0F5099BE1DE35AC8 CRC64;
MPVPASWPHP PGPFLLLTLL LGLTEVAGEE ELQMIQPEKL LLVTVGKTAT
LHCTVTSLLP VGPVLWFRGV GPGRELIYNQ KEGHFPRVTT VSDLTKRNNM
DFSIRISSIT PADVGTYYCV KFRKGSPENV EFKSGPGTEM ALGAKPSAPV
VLGPAARTTP EHTVSFTCES HGFSPRDITL KWFKNGNELS DFQTNVDPTG
QSVAYSIRST ARVVLDPWDV RSQVICEVAH VTLQGDPLRG TANLSEAIRV
PPTLEVTQQP MRVGNQVNVT CQVRKFYPQS LQLTWSENGN VCQRETASTL
TENKDGTYNW TSWFLVNISD QRDDVVLTCQ VKHDGQLAVS KRLALEVTVH
QKDQSSDATP GPASSLTALL LIAVLLGPIY VPWKQKT
UCSC uc002wfm.1; human. [Q9P1W8-1];

Category

Human SIRPG ELISA Kit