Human ATP1B1 ELISA Kit

Alternative name
Human Sodium/potassium-dependent ATPase subunit beta-1 ELISA Kit;Human ATP1B ELISA Kit;Human ATPase Na+/K+ transporting subunit beta 1 ELISA Kit;Human sodium/potassium-transporting ATPase subunit beta-1 ELISA Kit;Human ATPase, Na+/K+ transporting, beta 1 polypeptide ELISA Kit;Human Beta 1-subunit of Na(+),K(+)-ATPase ELISA Kit;Human Na, K-ATPase beta-1 polypeptide ELISA Kit;Human adenosinetriphosphatase ELISA Kit;Human sodium pump subunit beta-1 ELISA Kit;Human sodium-potassium ATPase subunit beta 1 (non-catalytic) ELISA Kit;Human sodium/potassium-dependent ATPase beta-1 subunit ELISA Kit;Human sodium/potassium-transporting ATPase beta-1 chain ELISA Kit;
Catalog
E035629
species
Human
GeneATP1B1
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATP1beta1. No significant cross-reactivity or interference between ATP1beta1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP1beta1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman ATP1B1 ELISA Kit allows for the in vitro quantitative determination of ATP1B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeuroscience
Product Description
specificalPrinciple of the assay: ATP1beta1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP1beta1 antibody and an ATP1beta1-HRP conjugate. The assay sample and buffer are incubated together with ATP1beta1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP1beta1 concentration since ATP1beta1 from samples and ATP1beta1-HRP conjugate compete for the anti-ATP1beta1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP1beta1 from the sample, fewer sites are left to bind ATP1beta1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP1beta1 concentration in each sample is interpolated from this standard curve.
Human Sodium/potassium-transporting ATPase subunit beta-1 Protein information
Uniprot ID AT1B1_HUMAN
Uniprot AC P05026; Q5TGZ3;
UniGene Hs.291196;
GeneID 481
KEGG hsa:481;
Human Sodium/potassium-transporting ATPase subunit beta-1 Protein SEQUENCE
SEQUENCE 303 AA; 35061 MW; 107D3C04394F2D11 CRC64;
MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF
IGTIQVMLLT ISEFKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA
YVLNIVRFLE KYKDSAQRDD MIFEDCGDVP SEPKERGDFN HERGERKVCR
FKLEWLGNCS GLNDETYGYK EGKPCIIIKL NRVLGFKPKP PKNESLETYP
VMKYNPNVLP VQCTGKRDED KDKVGNVEYF GLGNSPGFPL QYYPYYGKLL
QPKYLQPLLA VQFTNLTMDT EIRIECKAYG ENIGYSEKDR FQGRFDVKIE
VKS
UCSC uc001gfr.2; human. [P05026-1];

Category

Human ATP1B1 ELISA Kit