Human S1PR2 ELISA Kit

Alternative name
Human S1P receptor 2 ELISA Kit;Human S1P2 ELISA Kit;Human endothelial differentiation G-protein coupled receptor 5 ELISA Kit;Human sphingosine 1-phosphate receptor Edg-5 ELISA Kit;Human S1P receptor Edg-5 ELISA Kit;Human EDG5 ELISA Kit;Human AGR16 ELISA Kit;Human DFNB68 ELISA Kit;Human EDG-5 ELISA Kit;Human Gpcr13 ELISA Kit;Human H218 ELISA Kit;Human LPB2 ELISA Kit;Human sphingosine-1-phosphate receptor 2 ELISA Kit;Human sphingosine 1-phosphate receptor 2 ELISA Kit;Human CTD-2369P2.2 ELISA Kit;Human S1P receptor EDG5 ELISA Kit;Human deafness, autosomal recessive 68 ELISA Kit;Human endothelial differentiation, sphingolipid G-protein-coupled receptor, 5 ELISA Kit;
Catalog
E036441
species
Human
GeneS1PR2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.375ng/ml
Intended UseHuman S1PR2 ELISA Kit allows for the in vitro quantitative determination of S1PR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- S1PR2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- S1PR2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the S1PR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of S1PR2 can be calculated.
Human Sphingosine 1-phosphate receptor 2 Protein information
Uniprot ID S1PR2_HUMAN
Uniprot AC O95136; Q86UN8;
UniGene Hs.655405;
GeneID 9294
KEGG hsa:9294;
Human Sphingosine 1-phosphate receptor 2 Protein SEQUENCE
SEQUENCE 353 AA; 38867 MW; 8E37084284ABF7E8 CRC64;
MGSLYSEYLN PNKVQEHYNY TKETLETQET TSRQVASAFI VILCCAIVVE
NLLVLIAVAR NSKFHSAMYL FLGNLAASDL LAGVAFVANT LLSGSVTLRL
TPVQWFAREG SAFITLSASV FSLLAIAIER HVAIAKVKLY GSDKSCRMLL
LIGASWLISL VLGGLPILGW NCLGHLEACS TVLPLYAKHY VLCVVTIFSI
ILLAIVALYV RIYCVVRSSH ADMAAPQTLA LLKTVTIVLG VFIVCWLPAF
SILLLDYACP VHSCPILYKA HYFFAVSTLN SLLNPVIYTW RSRDLRREVL
RPLQCWRPGV GVQGRRRGGT PGHHLLPLRS SSSLERGMHM PTSPTFLEGN
TVV
UCSC uc002mnl.3; human.;

Category

Human S1PR2 ELISA Kit