Intended UseHuman SRXN1 ELISA Kit allows for the in vitro quantitative determination of SRXN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntended Uses: This immunoassay kit allows for the in vitro quantitative determination of humanSulfiredoxin-1concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toSulfiredoxin-1, During the reaction,Sulfiredoxin-1in the sample or standard competes with a fixed amount of biotin-labeledSulfiredoxin-1 for sites on a pre-coated Monoclonal antibody specific to Sulfiredoxin-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Sulfiredoxin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.