-
Catalog
E038306
-
species
Human
-
GeneT4
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of human T4. No significant cross-reactivity or interference between human T4 and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityThe minimum detectable dose of human T4 is typically less than 10 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human T4 concentration that could be differentiated from zero. It was determined the mean O
-
Intended UseHuman T4 ELISA Kit allows for the in vitro quantitative determination of T4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrincple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.
-