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Human GLI3 ELISA Kit

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  • Alternative name

    Human GLI3 form of 190 kDa ELISA Kit;Human GLI3-190 ELISA Kit;Human GLI3 full length protein ELISA Kit;Human GLI3FL ELISA Kit;Human GLI3 C-terminally truncated form ELISA Kit;Human GLI3 form of 83 kDa ELISA Kit;Human GLI3-83 ELISA Kit;Human ACLS ELISA Kit;Human GCPS ELISA Kit;Human PAP-A ELISA Kit;Human PAPA ELISA Kit;Human PAPA1 ELISA Kit;Human PAPB ELISA Kit;Human PHS ELISA Kit;Human PPDIV ELISA Kit;Human GLI family zinc finger 3 ELISA Kit;Human transcriptional activator GLI3 ELISA Kit;Human GLI-Kruppel family member GLI3 ELISA Kit;Human glioma-associated oncogene family zinc finger 3 ELISA Kit;Human oncogene GLI3 ELISA Kit;Human zinc finger protein GLI3 ELISA Kit;
  • Catalog
    E038867
  • species
    Human
  • GeneGLI3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GLI3. No significant cross-reactivity or interference between GLI3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLI3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman GLI3 ELISA Kit allows for the in vitro quantitative determination of GLI3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Intended Uses: This GLI3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of human GLI3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GLI3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GLI3 antibody and an GLI3-HRP conjugate. The assay sample and buffer are incubated together with GLI3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLI3 concentration since GLI3 from samples and GLI3-HRP conjugate compete for the anti-GLI3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLI3 from the sample, fewer sites are left to bind GLI3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLI3 concentration in each sample is interpolated from this standard curve.
  • Human Transcriptional repressor GLI3R Protein information
  • Uniprot ID GLI3_HUMAN
  • Uniprot AC P10071; A4D1W1; O75219; Q17RW4; Q75MT0; Q75MU9; Q9UDT5; Q9UJ39;
  • UniGene Hs.21509;
  • GeneID 2737
  • KEGG hsa:2737;
  • Human Transcriptional repressor GLI3R Protein SEQUENCE

  • SEQUENCE 1580 AA; 169863 MW; 423B7495FCE3C37C CRC64;

    MEAQSHSSTT TEKKKVENSI VKCSTRTDVS EKAVASSTTS NEDESPGQTY

    HRERRNAITM QPQNVQGLSK VSEEPSTSSD ERASLIKKEI HGSLPHVAEP

    SVPYRGTVFA MDPRNGYMEP HYHPPHLFPA FHPPVPIDAR HHEGRYHYDP

    SPIPPLHMTS ALSSSPTYPD LPFIRISPHR NPTAASESPF SPPHPYINPY

    MDYIRSLHSS PSLSMISATR GLSPTDAPHA GVSPAEYYHQ MALLTGQRSP

    YADIIPSAAT AGTGAIHMEY LHAMDSTRFS SPRLSARPSR KRTLSISPLS

    DHSFDLQTMI RTSPNSLVTI LNNSRSSSSA SGSYGHLSAS AISPALSFTY

    SSAPVSLHMH QQILSRQQSL GSAFGHSPPL IHPAPTFPTQ RPIPGIPTVL

    NPVQVSSGPS ESSQNKPTSE SAVSSTGDPM HNKRSKIKPD EDLPSPGARG

    QQEQPEGTTL VKEEGDKDES KQEPEVIYET NCHWEGCARE FDTQEQLVHH

    INNDHIHGEK KEFVCRWLDC SREQKPFKAQ YMLVVHMRRH TGEKPHKCTF

    EGCTKAYSRL ENLKTHLRSH TGEKPYVCEH EGCNKAFSNA SDRAKHQNRT

    HSNEKPYVCK IPGCTKRYTD PSSLRKHVKT VHGPEAHVTK KQRGDIHPRP

    PPPRDSGSHS QSRSPGRPTQ GALGEQQDLS NTTSKREECL QVKTVKAEKP

    MTSQPSPGGQ SSCSSQQSPI SNYSNSGLEL PLTDGGSIGD LSAIDETPIM

    DSTISTATTA LALQARRNPA GTKWMEHVKL ERLKQVNGMF PRLNPILPPK

    APAVSPLIGN GTQSNNTCSL GGPMTLLPGR SDLSGVDVTM LNMLNRRDSS

    ASTISSAYLS SRRSSGISPC FSSRRSSEAS QAEGRPQNVS VADSYDPIST

    DASRRSSEAS QSDGLPSLLS LTPAQQYRLK AKYAAATGGP PPTPLPNMER

    MSLKTRLALL GDALEPGVAL PPVHAPRRCS DGGAHGYGRR HLQPHDAPGH

    GVRRASDPVR TGSEGLALPR VPRFSSLSSC NPPAMATSAE KRSLVLQNYT

    RPEGGQSRNF HSSPCPPSIT ENVTLESLTM DADANLNDED FLPDDVVQYL

    NSQNQAGYEQ HFPSALPDDS KVPHGPGDFD APGLPDSHAG QQFHALEQPC

    PEGSKTDLPI QWNEVSSGSA DLSSSKLKCG PRPAVPQTRA FGFCNGMVVH

    PQNPLRSGPA GGYQTLGENS NPYGGPEHLM LHNSPGSGTS GNAFHEQPCK

    APQYGNCLNR QPVAPGALDG ACGAGIQASK LKSTPMQGSG GQLNFGLPVA

    PNESAGSMVN GMQNQDPVGQ GYLAHQLLGD SMQHPGAGRP GQQMLGQISA

    TSHINIYQGP ESCLPGAHGM GSQPSSLAVV RGYQPCASFG GSRRQAMPRD

    SLALQSGQLS DTSQTCRVNG IKMEMKGQPH PLCSNLQNYS GQFYDQTVGF

    SQQDTKAGSF SISDASCLLQ GTSAKNSELL SPGANQVTST VDSLDSHDLE

    GVQIDFDAII DDGDHSSLMS GALSPSIIQN LSHSSSRLTT PRASLPFPAL

    SMSTTNMAIG DMSSLLTSLA EESKFLAVMQ

  • UCSC uc011kbh.3; human.;



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