Human UBE3A ELISA Kit

Alternative name
Human E6AP ubiquitin-protein ligase ELISA Kit;Human HECT-type ubiquitin transferase E3A ELISA Kit;Human human papillomavirus E6-associated protein ELISA Kit;Human oncogenic protein-associated protein E6-AP ELISA Kit;Human renal carcinoma antigen NY-REN-54 ELISA Kit;Human E6AP ELISA Kit;Human EPVE6AP ELISA Kit;Human HPVE6A ELISA Kit;Human ANCR ELISA Kit;Human AS ELISA Kit;Human E6-AP ELISA Kit;Human ubiquitin protein ligase E3A ELISA Kit;Human ubiquitin-protein ligase E3A ELISA Kit;Human CTCL tumor antigen se37-2 ELISA Kit;Human human papilloma virus E6-associated protein ELISA Kit;
Catalog
E040211
species
Human
GeneUBE3A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human UBE3A. No significant cross-reactivity or interference between Human UBE3A and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human UBE3A and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.188ng/mL
Intended UseHuman UBE3A ELISA Kit allows for the in vitro quantitative determination of UBE3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of UBE3A in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human UBE3A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE3A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human UBE3A. You can calculate the concentration of Human UBE3A in the samples by comparing the OD of the samples to the standard curve.
Human Ubiquitin-protein ligase E3A Protein information
Uniprot ID UBE3A_HUMAN
Uniprot AC Q9UEP8; Q9UEP9;
UniGene Hs.598862;
GeneID 7337
KEGG hsa:7337;
Human Ubiquitin-protein ligase E3A Protein SEQUENCE
SEQUENCE 875 AA; 100688 MW; F80F0502B3B3838A CRC64;
MEKLHQCYWK SGEPQSDDIE ASRMKRAAAK HLIERYYHQL TEGCGNEACT
NEFCASCPTF LRMDNNAAAI KALELYKINA KLCDPHPSKK GASSAYLENS
KGAPNNSCSE IKMNKKGARI DFKDVTYLTE EKVYEILELC REREDYSPLI
RVIGRVFSSA EALVQSFRKV KQHTKEELKS LQAKDEDKDE DEKEKAACSA
AAMEEDSEAS SSRIGDSSQG DNNLQKLGPD DVSVDIDAIR RVYTRLLSNE
KIETAFLNAL VYLSPNVECD LTYHNVYSRD PNYLNLFIIV MENRNLHSPE
YLEMALPLFC KAMSKLPLAA QGKLIRLWSK YNADQIRRMM ETFQQLITYK
VISNEFNSRN LVNDDDAIVA ASKCLKMVYY ANVVGGEVDT NHNEEDDEEP
IPESSELTLQ ELLGEERRNK KGPRVDPLET ELGVKTLDCR KPLIPFEEFI
NEPLNEVLEM DKDYTFFKVE TENKFSFMTC PFILNAVTKN LGLYYDNRIR
MYSERRITVL YSLVQGQQLN PYLRLKVRRD HIIDDALVRL EMIAMENPAD
LKKQLYVEFE GEQGVDEGGV SKEFFQLVVE EIFNPDIGMF TYDESTKLFW
FNPSSFETEG QFTLIGIVLG LAIYNNCILD VHFPMVVYRK LMGKKGTFRD
LGDSHPVLYQ SLKDLLEYEG NVEDDMMITF QISQTDLFGN PMMYDLKENG
DKIPITNENR KEFVNLYSDY ILNKSVEKQF KAFRRGFHMV TNESPLKYLF
RPEEIELLIC GSRNLDFQAL EETTEYDGGY TRDSVLIREF WEIVHSFTDE
QKRLFLQFTT GTDRAPVGGL GKLKMIIAKN GPDTERLPTS HTCFNVLLLP
EYSSKEKLKE RLLKAITYAK GFGML
UCSC uc001zaq.4; human. [Q05086-1];

Category

Human UBE3A ELISA Kit