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Human PFKFB3 ELISA Kit

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  • Alternative name

    Human 6PF-2-K/Fru-2,6-P2ase 3 ELISA Kit;Human PFK/FBPase 3 ELISA Kit;Human 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme ELISA Kit;Human renal carcinoma antigen NY-REN-56 ELISA Kit;Human iPFK-2 ELISA Kit;Human IPFK2 ELISA Kit;Human PFK2 ELISA Kit;Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 ELISA Kit;Human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 ELISA Kit;Human 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase ELISA Kit;Human fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase ELISA Kit;Human inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase ELISA Kit;
  • Catalog
    E042120
  • species
    Human
  • GenePFKFB3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PFKFB3. No significant cross-reactivity or interference between PFKFB3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PFKFB3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PFKFB3 ELISA Kit allows for the in vitro quantitative determination of PFKFB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: PFKFB3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFKFB3 antibody and an PFKFB3-HRP conjugate. The assay sample and buffer are incubated together with PFKFB3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFKFB3 concentration since PFKFB3 from samples and PFKFB3-HRP conjugate compete for the anti-PFKFB3 antibody binding site. Since the number of sites is limited, as more sites are occupied by PFKFB3 from the sample, fewer sites are left to bind PFKFB3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFKFB3 concentration in each sample is interpolated from this standard curve.
  • Human Fructose-2,6-bisphosphatase Protein information
  • Uniprot ID F263_HUMAN
  • Uniprot AC Q16875; B7Z955; O43622; O75902; Q5VX15; Q5VX18; Q5VX19;
  • UniGene Hs.195471;
  • GeneID 5209
  • KEGG hsa:5209;
  • Human Fructose-2,6-bisphosphatase Protein SEQUENCE

  • SEQUENCE 520 AA; 59609 MW; A7675A4ADC376879 CRC64;

    MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY

    ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMKVRK

    QCALAALRDV KSYLAKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF

    IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR ISCYEASYQP

    LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY

    LCRHGENEHN LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ

    LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ

    DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL

    DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVC THRERSEDAK

    KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT

    QLPGQNMKGS RSSADSSRKH

  • UCSC uc001ijd.4; human. [Q16875-1];



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