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Mouse Actg2 ELISA Kit

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  • Alternative name

    Mouse alpha-actin-3 ELISA Kit;Mouse gamma-2-actin ELISA Kit;Mouse smooth muscle gamma-actin ELISA Kit;Mouse ACTA3 ELISA Kit;Mouse Actsg ELISA Kit;Mouse Act-4 ELISA Kit;Mouse Act4 ELISA Kit;Mouse SMGA ELISA Kit;Mouse actin, gamma 2, smooth muscle, enteric ELISA Kit;Mouse actin, gamma-enteric smooth muscle ELISA Kit;
  • Catalog
    E042555
  • species
    Mouse
  • GeneActg2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACTG2. No significant cross-reactivity or interference between ACTG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTG2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 mug/mL.
  • Intended UseMouse Actg2 ELISA Kit allows for the in vitro quantitative determination of Actg2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: ACTG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTG2 antibody and an ACTG2-HRP conjugate. The assay sample and buffer are incubated together with ACTG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTG2 concentration since ACTG2 from samples and ACTG2-HRP conjugate compete for the anti-ACTG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTG2 from the sample, fewer sites are left to bind ACTG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTG2 concentration in each sample is interpolated from this standard curve.
  • Mouse Actin, gamma-enteric smooth muscle Protein information
  • Uniprot ID ACTH_MOUSE
  • Uniprot AC P63268; P12718;
  • UniGene Mm.292865;
  • GeneID 11468
  • KEGG mmu:11468;
  • Mouse Actin, gamma-enteric smooth muscle Protein SEQUENCE

  • SEQUENCE 376 AA; 41877 MW; 6EC08CD5EEAD445E CRC64;

    MCEEETTALV CDNGSGLCKA GFAGDDAPRA VFPSIVGRPR HQGVMVGMGQ

    KDSYVGDEAQ SKRGILTLKY PIEHGIITNW DDMEKIWHHS FYNELRVAPE

    EHPTLLTEAP LNPKANREKM TQIMFETFNV PAMYVAIQAV LSLYASGRTT

    GIVLDSGDGV THNVPIYEGY ALPHAIMRLD LAGRDLTDYL MKILTERGYS

    FVTTAEREIV RDIKEKLCYV ALDFENEMAT AASSSSLEKS YELPDGQVIT

    IGNERFRCPE TLFQPSFIGM ESAGIHETTY NSIMKCDIDI RKDLYANNVL

    SGGTTMYPGI ADRMQKEITA LAPSTMKIKI IAPPERKYSV WIGGSILASL

    STFQQMWISK PEYDEAGPSI VHRKCF

  • UCSC uc009cnr.1; mouse.;



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