Mouse Aars2 ELISA Kit

Alternative name
Mouse Alanyl-tRNA synthetase ELISA Kit;Mouse alaRS ELISA Kit;Mouse Aarsl ELISA Kit;Mouse Gm89 ELISA Kit;Mouse Kiaa1270 ELISA Kit;Mouse alanyl-tRNA synthetase 2, mitochondrial ELISA Kit;Mouse alanine--tRNA ligase, mitochondrial ELISA Kit;Mouse alanyl-tRNA synthetase 2, mitochondrial (putative) ELISA Kit;Mouse alanyl-tRNA synthetase like ELISA Kit;Mouse alanyl-tRNA synthetase, mitochondrial ELISA Kit;Mouse probable alanyl-tRNA synthetase, mitochondrial ELISA Kit;
Catalog
E043143
species
Mouse
GeneAars2
SpecificityThis assay has high sensitivity and excellent specificity for detection of AARS2. No significant cross-reactivity or interference between AARS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AARS2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Aars2 ELISA Kit allows for the in vitro quantitative determination of Aars2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: AARS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AARS2 antibody and an AARS2-HRP conjugate. The assay sample and buffer are incubated together with AARS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AARS2 concentration since AARS2 from samples and AARS2-HRP conjugate compete for the anti-AARS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AARS2 from the sample, fewer sites are left to bind AARS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AARS2 concentration in each sample is interpolated from this standard curve.
Mouse Alanine--tRNA ligase, mitochondrial Protein information
Uniprot ID SYAM_MOUSE
Uniprot AC Q14CH7; Q68FH3; Q69ZM9;
UniGene Mm.329063;
GeneID 224805
KEGG mmu:224805;
Mouse Alanine--tRNA ligase, mitochondrial Protein SEQUENCE
SEQUENCE 980 AA; 106783 MW; 7734BC58CDB5463D CRC64;
MAVALAAAAG KLRRAIGRSC PWQPFSTEPG PPHGAAVRDA FLSFFRDRHG
HRLVPSATVR PRGDPSLLFV NAGMNQFKPI FLGTVDPRSE MAGFRRVVNS
QKCVRAGGRH NDLEDVGRDL SHHTFFEMLG NWAFGGEYFK EEACSMAWEL
LTQVYGIPED RLWVSYFSGD SQTGLDPDLE TRDIWLSLGV PASRVLSFGP
QENFWEMGDT GPCGPCTEIH YDLAGGVGSP QLVELWNLVF MQHYREADGS
LQLLPQRHVD TGMGLERLVA VLQGKRSTYD TDLFSPLLDA IHQSCGAPPY
SGRVGAADEG RIDTAYRVVA DHIRTLSVCI ADGVSPGMSG APLVLRRILR
RAVRYSTEVL QAPPGFLGSL VPVVVETLGS AYPELEKNSV KIASLVSEDE
AAFLASLQRG RRIIDRTVKR LGPSDLFPAE VAWSLSLSGN LGIPLDLVEL
MLEEKGVKLD TAGLEQLAQK EAQHRAQQAE ADQEDRLCLD VHALEELHRQ
GIPTTDDSPK YNYTLHPNGD YEFGLCEARV LQLYSETGTA VASVGAGQRC
GLLLDRTNFY AEQGGQASDR GYLVRTGQQD MLFPVAGAQL CGGFILHEAM
APERLQVGDQ VQLYVDKAWR MGCMVKHTAT HLLSWALRQT LGPTTEQRGS
HLNPERLRFD VATQTLLTTE QLRTVESYVQ EVVGQDKPVF MEEVPLAHTA
RIPGLRSLDE VYPDPVRVVS VGVPVAHALG PASQAAMHTS VELCCGTHLL
STGAVGDLVI IGERQLVKGI TRLLAITGEQ AQQAREVGQS LSQEVEAASE
RLSQGSRDLP EAHRLSKDIG RLTEVAESAV IPQWQRQELQ TTLKMLQRRA
NTAIRKLEKG QATEKSQELL KRHSEGPLIV DTVSAESLSV LVKVVRQLCK
QAPSISVLLL SPQPTGSVLC ACQVAQDATP TFTAEAWALA VCSHMGGKAW
GSRVVAQGTG HTADLEAALG TARAYALSQL
UCSC uc008cqr.1; mouse.;

Category

Mouse Aars2 ELISA Kit