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Alternative name
Mouse V-ATPase subunit e 1 ELISA Kit;Mouse vacuolar proton pump subunit e 1 ELISA Kit;Mouse Atp6v0e ELISA Kit;Mouse Atp6k ELISA Kit;Mouse Atp6v0e1 ELISA Kit;Mouse M9.2 ELISA Kit;Mouse ATPase, H+ transporting, lysosomal V0 subunit E ELISA Kit;Mouse V-type proton ATPase subunit e 1 ELISA Kit;Mouse ATPase, H+ transporting lysosomal (vacuolar proton pump), 9.2 kDa ELISA Kit;Mouse ATPase, H+ transporting, V0 subunit e ELISA Kit;Mouse ATPase, H+ transporting, lysosomal 9kD V0 subunit E ELISA Kit;Mouse lysosomal 9kDa ELISA Kit;
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Catalog
E045237
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species
Mouse
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GeneAtp6v0e1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse ATP6V0E1. No significant cross-reactivity or interference between mouse ATP6V0E1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse ATP6V0E1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
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Intended UseMouse Atp6v0e1 ELISA Kit allows for the in vitro quantitative determination of Atp6v0e1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATP6V0E1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATP6V0E1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP6V0E1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP6V0E1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Mouse V-type proton ATPase subunit e 1 Protein information
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Uniprot ID
VA0E1_MOUSE
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Uniprot AC
Q9CQD8;
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UniGene
Mm.22602;
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GeneID
11974
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KEGG
mmu:11974;
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Mouse V-type proton ATPase subunit e 1 Protein SEQUENCE
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SEQUENCE 81 AA; 9340 MW; 9A47F165D72B2A2D CRC64;
MAYHGLTVPL IVMSVFWGFV GLLVPWFIPK GPNRGVIITM LVTCSVCCYL
FWLIAILAQL
NPLFGPQLKN ETIWYLKYHW P
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UCSC
uc008beh.1; mouse.;
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