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Alternative name
Mouse 1810056O20Rik ELISA Kit;Mouse bolA-like 3 (E. coli) ELISA Kit;Mouse bolA-like protein 3 ELISA Kit;
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Catalog
E045937
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species
Mouse
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GeneBola3
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SpecificityThis assay has high sensitivity and excellent specificity for detection of BOLA3. No significant cross-reactivity or interference between BOLA3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BOLA3 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse Bola3 ELISA Kit allows for the in vitro quantitative determination of Bola3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Categories/FamilyCell Biology
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Product Description
specificalPrinciple of the assay: BOLA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BOLA3 antibody and an BOLA3-HRP conjugate. The assay sample and buffer are incubated together with BOLA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BOLA3 concentration since BOLA3 from samples and BOLA3-HRP conjugate compete for the anti-BOLA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by BOLA3 from the sample, fewer sites are left to bind BOLA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BOLA3 concentration in each sample is interpolated from this standard curve.
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Mouse BolA-like protein 3 Protein information
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Uniprot ID
BOLA3_MOUSE
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Uniprot AC
Q8CEI1;
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UniGene
Mm.391470;
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GeneID
78653
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KEGG
mmu:78653;
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Mouse BolA-like protein 3 Protein SEQUENCE
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SEQUENCE 110 AA; 12228 MW; 84DA4ECA50547C56 CRC64;
MAAWGPAAAA PLLRGSRGLP LWHCAQRMFA SQTEGELKVT QVLKEKFPRA
TAIQVTDISG
GCGAMYEIKI ESEEFKEKRT VQQHQMVNQA LKEEIKGMHG
LRIFTSVPKC
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UCSC
uc009cnh.1; mouse.;
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