Human TUBB ELISA Kit

Alternative name
Human tubulin beta-5 chain ELISA Kit;Human TUBB5 ELISA Kit;Human CDCBM6 ELISA Kit;Human CSCSC1 ELISA Kit;Human M40 ELISA Kit;Human OK/SW-cl.56 ELISA Kit;Human TUBB1 ELISA Kit;Human tubulin beta class I ELISA Kit;Human tubulin beta chain ELISA Kit;Human beta Ib tubulin ELISA Kit;Human tubulin beta-1 chain ELISA Kit;Human tubulin, beta polypeptide ELISA Kit;
Catalog
E004719
species
Human
GeneTUBB
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman TUBB ELISA Kit allows for the in vitro quantitative determination of TUBB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: TUBbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TUBbeta antibody and an TUBbeta-HRP conjugate. The assay sample and buffer are incubated together with TUBbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TUBbeta concentration since TUBbeta from samples and TUBbeta-HRP conjugate compete for the anti-TUBbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by TUBbeta from the sample, fewer sites are left to bind TUBbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TUBbeta concentration in each sample is interpolated from this standard curve.
Human Tubulin beta chain Protein information
Uniprot ID TBB5_HUMAN
Uniprot AC P07437; P05218; Q8WUC1; Q9CY33;
UniGene Hs.636480;
GeneID 203068
KEGG hsa:203068;
Human Tubulin beta chain Protein SEQUENCE
SEQUENCE 444 AA; 49671 MW; 1E6CD0A36773A103 CRC64;
MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLDRISVY
YNEATGGKYV PRAILVDLEP GTMDSVRSGP FGQIFRPDNF VFGQSGAGNN
WAKGHYTEGA ELVDSVLDVV RKEAESCDCL QGFQLTHSLG GGTGSGMGTL
LISKIREEYP DRIMNTFSVV PSPKVSDTVV EPYNATLSVH QLVENTDETY
CIDNEALYDI CFRTLKLTTP TYGDLNHLVS ATMSGVTTCL RFPGQLNADL
RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVPELT QQVFDAKNMM
AACDPRHGRY LTVAAVFRGR MSMKEVDEQM LNVQNKNSSY FVEWIPNNVK
TAVCDIPPRG LKMAVTFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG
EGMDEMEFTE AESNMNDLVS EYQQYQDATA EEEEDFGEEA EEEA

Category

Human TUBB ELISA Kit