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Alternative name
C-C motif chemokine 16 ELISA KIT; Chemokine CC-4 ELISA KIT; HCC-4 ELISA KIT; Chemokine LEC ELISA KIT; IL-10-inducible chemokine ELISA KIT; LCC-1 ELISA KIT; Liver-expressed chemokine ELISA KIT; Lymphocyte and monocyte chemoattractant ELISA KIT; LMC ELISA KIT; Monotactin-1 ELISA KIT; MTN-1 ELISA KIT; NCC-4 ELISA KIT; Small-inducible cytokine A16CCL16 ELISA KIT; ILINCK ELISA KIT; NCC4 ELISA KIT; SCYA16 ELISA KIT; HCC-4 ELISA KIT; LMC ELISA KIT; MTN-1 ELISA KIT
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Catalog
E048138
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species
Mouse
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GeneCCL16
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse CCL16 ELISA Kit allows for the in vitro quantitative determination of CCL16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalIntended Uses: This CCL16 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCCL16 in the sample, thisCCL16 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCCL16 concentration. The concentration ofCCL16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This CCL16 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCCL16. Standards or samples are then added to the microtiter plate wells andCCL16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCCL16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCCL16 are added to each well to "sandwich" theCCL16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCCL16 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
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