Human CFB ELISA Kit

Alternative name
Human C3/C5 convertase ELISA Kit;Human Glycine-rich beta glycoprotein ELISA Kit;Human GBG ELISA Kit;Human PBF2 ELISA Kit;Human properdin factor B ELISA Kit;Human BF ELISA Kit;Human BFD ELISA Kit;Human AHUS4 ELISA Kit;Human ARMD14 ELISA Kit;Human CFAB ELISA Kit;Human CFBD ELISA Kit;Human FB ELISA Kit;Human FBI12 ELISA Kit;Human H2-Bf ELISA Kit;Human complement factor B ELISA Kit;Human B-factor, properdin ELISA Kit;Human C3 proaccelerator ELISA Kit;Human C3 proactivator ELISA Kit;Human glycine-rich beta-glycoprotein ELISA Kit;
Catalog
E007879
species
Human
GeneCFB
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CFB. No significant cross-reactivity or interference between Human CFB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CFB and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.875ng/mL
Intended UseHuman CFB ELISA Kit allows for the in vitro quantitative determination of CFB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFB in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CFB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CFB. You can calculate the concentration of Human CFB in the samples by comparing the OD of the samples to the standard curve.
Human Complement factor B Bb fragment Protein information
Uniprot ID CFAB_HUMAN
Uniprot AC Q9BTF5; Q9BX92;
UniGene Hs.69771;
GeneID 629
KEGG hsa:629;
Human Complement factor B Bb fragment Protein SEQUENCE
SEQUENCE 764 AA; 85533 MW; 8BB6C101CC6AC200 CRC64;
MGSNLSPQLC LMPFILGLLS GGVTTTPWSL ARPQGSCSLE GVEIKGGSFR
LLQEGQALEY VCPSGFYPYP VQTRTCRSTG SWSTLKTQDQ KTVRKAECRA
IHCPRPHDFE NGEYWPRSPY YNVSDEISFH CYDGYTLRGS ANRTCQVNGR
WSGQTAICDN GAGYCSNPGI PIGTRKVGSQ YRLEDSVTYH CSRGLTLRGS
QRRTCQEGGS WSGTEPSCQD SFMYDTPQEV AEAFLSSLTE TIEGVDAEDG
HGPGEQQKRK IVLDPSGSMN IYLVLDGSDS IGASNFTGAK KCLVNLIEKV
ASYGVKPRYG LVTYATYPKI WVKVSEADSS NADWVTKQLN EINYEDHKLK
SGTNTKKALQ AVYSMMSWPD DVPPEGWNRT RHVIILMTDG LHNMGGDPIT
VIDEIRDLLY IGKDRKNPRE DYLDVYVFGV GPLVNQVNIN ALASKKDNEQ
HVFKVKDMEN LEDVFYQMID ESQSLSLCGM VWEHRKGTDY HKQPWQAKIS
VIRPSKGHES CMGAVVSEYF VLTAAHCFTV DDKEHSIKVS VGGEKRDLEI
EVVLFHPNYN INGKKEAGIP EFYDYDVALI KLKNKLKYGQ TIRPICLPCT
EGTTRALRLP PTTTCQQQKE ELLPAQDIKA LFVSEEEKKL TRKEVYIKNG
DKKGSCERDA QYAPGYDKVK DISEVVTPRF LCTGGVSPYA DPNTCRGDSG
GPLIVHKRSR FIQVGVISWG VVDVCKNQKR QKQVPAHARD FHINLFQVLP
WLKEKLQDED LGFL
UCSC uc003nyj.5; human. [P00751-1];

Category

Human CFB ELISA Kit