Mouse Ccdc80 ELISA Kit

Alternative name
Mouse up-regulated in BRS-3 deficient mouse ELISA Kit;Mouse Urb ELISA Kit;Mouse 2610001E17Rik ELISA Kit;Mouse DRO1 ELISA Kit;Mouse Ssg1 ELISA Kit;Mouse coiled-coil domain containing 80 ELISA Kit;Mouse coiled-coil domain-containing protein 80 ELISA Kit;Mouse steroid sensitive gene 1 ELISA Kit;
Catalog
E048997
species
Mouse
GeneCcdc80
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCDC80. No significant cross-reactivity or interference between CCDC80 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCDC80 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Ccdc80 ELISA Kit allows for the in vitro quantitative determination of Ccdc80 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This CCDC80 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CCDC80. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CCDC80 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCDC80 antibody and an CCDC80-HRP conjugate. The assay sample and buffer are incubated together with CCDC80-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCDC80 concentration since CCDC80 from samples and CCDC80-HRP conjugate compete for the anti-CCDC80 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCDC80 from the sample, fewer sites are left to bind CCDC80-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCDC80 concentration in each sample is interpolated from this standard curve.
Mouse Coiled-coil domain-containing protein 80 Protein information
Uniprot ID CCD80_MOUSE
Uniprot AC Q9D6Z4;
UniGene Mm.181074;
GeneID 67896
KEGG mmu:67896;
Mouse Coiled-coil domain-containing protein 80 Protein SEQUENCE
SEQUENCE 949 AA; 107613 MW; 25FB84D3BED55DF5 CRC64;
MMWKMGPHFT TLLAMWLVCG SASHSPALDS DSHTGRKVPL VSPISSRSAR
YLRHTGRSGG VEKSTQEEPN PQPFQRRKSV PVLRLAHPTM RPPPSGINGV
PVRPEVRPIA RSSAREMVRD EGSSARTRML RFPSGSSSPN ILASFAGKNR
VWVISAPHAS EGYYRLMMSL LKDDVYCELA ERHIQQIVLF HQAGEEGGKV
RRITNEGQIL EQPLDPNLIP KLMSFLKLEK GKFSMVLLKK TLQVEERYPY
PVRLEAMYEV IDQGPIRRIE KIRQKGFVQK CKASGIEGHV VQEGNEGGGG
AGGTGLGGDK RKEDPRRTQV HPTREAPRKQ ATSKAATPQP PPTPRATTLP
PAPVTTATRA TSRVVTIAAR PTTTTAYPAT QRPWTSRLHP FSVSHRPPAT
AEVTTARGPS VSEQLYPLPR KEQQREKPQA TRRPSKATNY GSFTATPPPT
LWEVSARVVG TSRFRDNRTD KREHGHQDPN AVPGPHKPVK GKLPKKKDRI
LSNEYEDKYD LSQPTSSQGE EERQVDSVPS QNAKESKKLE KLEKPEKEKK
KKGKSAKQDK LLKSEKQAKK AEKKTKQEKD KNKKKKAGKT EQDDNQKPTA
KHLAPSPKKS VADLLGSFEG KRRLLLITTP KAENNMYVQQ RDEYLESFCK
MATRRISVVT IFGPVNNSSM KIDHFQLDNE KPMRVVDDDD LVDQHLISEL
RKEYGMTYDD FFMVLTDVDL RVKQYYEVPI AMKSVFDLID TFQSRIKDME
KQKKEGIACK EDKRQSLENF LSRFRWRRRL LVISAPNDED WAYSQQLSAL
NGQACNFGLR HITILKLLGV GEEVGGVLEL FPINGSSIVE REDVPAHLVK
DIRNYFQVSP EYFSMLLVGK DGNVKSWYPS PMWSMVIVYD LIDSMQLRRQ
EMAIQQSLGM RCPEDEYAGY GYHSYHQGYQ DGYQDDYRHH ESYHHGYPY
UCSC uc007zif.1; mouse.;

Category

Mouse Ccdc80 ELISA Kit

Mouse up-regulated in BRS-3 deficient mouse ELISA Kit;Mouse Urb ELISA Kit;Mouse 2610001E17Rik ELISA Kit;Mouse DRO1 ELISA Kit;Mouse Ssg1 ELISA Kit;Mouse coiled-coil domain containing 80 ELISA Kit;Mouse coiled-coil domain-containing protein 80 ELISA Kit;Mouse steroid sensitive gene 1 ELISA Kit;