Mouse Cyp19a1 ELISA Kit

Alternative name
Mouse CYPXIX ELISA Kit;Mouse cytochrome P-450AROM ELISA Kit;Mouse cytochrome P450 19A1 ELISA Kit;Mouse estrogen synthase ELISA Kit;Mouse Arom ELISA Kit;Mouse Cyp19 ELISA Kit;Mouse Ar ELISA Kit;Mouse ArKO ELISA Kit;Mouse Int-5 ELISA Kit;Mouse Int5 ELISA Kit;Mouse p450arom ELISA Kit;Mouse cytochrome P450, family 19, subfamily a, polypeptide 1 ELISA Kit;Mouse aromatase ELISA Kit;Mouse cytochrome P450, 19, aromatase ELISA Kit;
Catalog
E050285
species
Mouse
GeneCyp19a1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseMouse Cyp19a1 ELISA Kit allows for the in vitro quantitative determination of Cyp19a1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Cyp19a1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Cyp19a1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cyp19a1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Cyp19a1 can be calculated.
Mouse Aromatase Protein information
Uniprot ID CP19A_MOUSE
Uniprot AC P28649;
UniGene Mm.478781; Mm.5199;
GeneID 13075
KEGG mmu:13075;
Mouse Aromatase Protein SEQUENCE
SEQUENCE 503 AA; 58015 MW; 737400389D1AEFF1 CRC64;
MFLEMLNPMQ YNVTIMVPET VTVSAMPLLL IMGLLLLIWN CESSSSIPGP
GYCLGIGPLI SHGRFLWMGI GSACNYYNKM YGEFMRVWIS GEETLIISKS
SSMFHVMKHS HYISRFGSKR GLQCIGMHEN GIIFNNNPSL WRTIRPFFMK
ALTGPGLVRM VEVCVESIKQ HLDRLGEVTD TSGYVDVLTL MRHIMLDTSN
MLFLGIPLDE SAIVKKIQGY FNAWQALLIK PNIFFKISWL YRKYERSVKD
LKDEIAVLVE KKRHKVSTAE KLEDCMDFAT DLIFAERRGD LTKENVNQCI
LEMLIAAPDT MSVTLYFMLL LVAEYPEVEA AILKEIHTVV GDRDIKIEDI
QNLKVVENFI NESMRYQPVV DLVMRRALED DVIDGYPVKK GTNIILNIGR
MHRLEYFPKP NEFTLENFEK NVPYRYFQPF GFGPRGCAGK YIAMVMMKVV
LVTLLRRFQV KTLQKRCIEN IPKKNDLSLH PNEDRHLVEI IFSPRNSDKY
LQQ
UCSC uc009pmu.1; mouse.;

Category

Mouse Cyp19a1 ELISA Kit