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Mouse Eloa ELISA Kit

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  • Alternative name

    Mouse EloA ELISA Kit;Mouse elongin 110 kDa subunit ELISA Kit;Mouse RNA polymerase II transcription factor SIII subunit A1 ELISA Kit;Mouse SIII p110 ELISA Kit;Mouse transcription elongation factor B polypeptide 3 ELISA Kit;Mouse Tceb3 ELISA Kit;Mouse 110kDa ELISA Kit;Mouse AA408125 ELISA Kit;Mouse Tceb3a ELISA Kit;Mouse elongin A ELISA Kit;
  • Catalog
    E051695
  • species
    Mouse
  • GeneEloa
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse ELOA. No significant cross-reactivity or interference between Mouse ELOA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse ELOA and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Eloa ELISA Kit allows for the in vitro quantitative determination of Eloa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ELOA in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ELOA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse ELOA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ELOA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ELOA. You can calculate the concentration of Mouse ELOA in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Elongin-A Protein information
  • Uniprot ID ELOA1_MOUSE
  • Uniprot AC Q8CB77; Q3UI38; Q80VB2; Q9R0Q5;
  • UniGene Mm.27663;
  • GeneID 27224
  • KEGG mmu:27224;
  • Mouse Elongin-A Protein SEQUENCE

  • SEQUENCE 773 AA; 87161 MW; 7F5B33C4B6E87561 CRC64;

    MAAESALQVV EKLQARLAAN PDPKKLLKYL KKLSILPITV DILVETGVGK

    TVNSFRKHEQ VGNFARDLVA QWKKLVPVER NSEAEDQDFE KNNSRKRPRD

    ALQREEELEG NYQESWKPSG SRSYSPEHRQ KKHKKLSEPE RPHKVAHSHE

    KRDERKRCHK VSPPYSSDPE SSDYGHVQSP PPSSPHQMYT DLSRSPEEDQ

    EPIISHQKPG KVHSNTFQDR LGVSHLGEQG KGAVSHHKQH RSSHKEKHPA

    DAREDEKISA VSREKSHKAS SKEESRRLLS GDSAKEKLPS SVVKKDKDRE

    GSSLKKKFSP ALDVASDNHF KKPKHKDSEK AKSDKNKQSV DGVDSGRGTG

    DPLPKAKEKV PNHLKAQEGK VRTNADGKSA GPLHPKAEET DVDDEFERPT

    MSFESYLSYD QPRKKKKKVV KTSSTALGEK GLKKKDSKST SKNLNSAQKL

    PKVNENKSEK LQPAGAEPTR PRKVPTDVLP ALPDIPLPAI HANYRPLPSL

    ELIPSFQPKR KAFSSPQEEE EAGFTGRRMN SKMQVYSGSK CAYLPKMMTL

    HQQCIRVLKN NIDSIFEVGG VPYSVLEPVL ERCTPDQLYR IEECNHVLIE

    ETDQLWKVHC HRDFKEERPE EYESWREMYL RLQDAREQRL RLLTNNIRSA

    HANKPKGRQA KMAFVNSVAK PPRDVRRRQE KFGTGGAAVP EKVRIKPAPY

    TTGSSHVPAS NSSSNFHSSP EELAYDGPST SSAHLAPVAS SSVSYDPRKP

    AVKKIAPMMA KTIKAFKNRF SRR

  • UCSC uc008vhp.1; mouse.;



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