Mouse Epor ELISA Kit

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  • Alternative name

    Mouse EPO-R ELISA Kit;Mouse erythropoietin receptor ELISA Kit;

  • Catalog
    E052058
  • species
    Mouse
  • GeneEpor
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse EPOR. No significant cross-reactivity or interference between Mouse EPOR and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse EPOR and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Epor ELISA Kit allows for the in vitro quantitative determination of Epor , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EPOR in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EPOR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse EPOR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EPOR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse EPOR. You can calculate the concentration of Mouse EPOR in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Erythropoietin receptor Protein information
  • Uniprot ID EPOR_MOUSE
  • Uniprot AC P14753; Q63852;
  • UniGene Mm.2653;
  • GeneID 13857
  • KEGG mmu:13857;
  • Mouse Erythropoietin receptor Protein SEQUENCE
  • SEQUENCE 507 AA; 55194 MW; 067657A2E26451CA CRC64;

    MDKLRVPLWP RVGPLCLLLA GAAWAPSPSL PDPKFESKAA LLASRGSEEL

    LCFTQRLEDL VCFWEEAASS GMDFNYSFSY QLEGESRKSC SLHQAPTVRG

    SVRFWCSLPT ADTSSFVPLE LQVTEASGSP RYHRIIHINE VVLLDAPAGL

    LARRAEEGSH VVLRWLPPPG APMTTHIRYE VDVSAGNRAG GTQRVEVLEG

    RTECVLSNLR GGTRYTFAVR ARMAEPSFSG FWSAWSEPAS LLTASDLDPL

    ILTLSLILVL ISLLLTVLAL LSHRRTLQQK IWPGIPSPES EFEGLFTTHK

    GNFQLWLLQR DGCLWWSPGS SFPEDPPAHL EVLSEPRWAV TQAGDPGADD

    EGPLLEPVGS EHAQDTYLVL DKWLLPRTPC SENLSGPGGS VDPVTMDEAS

    ETSSCPSDLA SKPRPEGTSP SSFEYTILDP SSQLLCPRAL PPELPPTPPH

    LKYLYLVVSD SGISTDYSSG GSQGVHGDSS DGPYSHPYEN SLVPDSEPLH

    PGYVACS

  • UCSC uc009one.1; mouse. [P14753-2];



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