Mouse Gapdh ELISA Kit

Alternative name
Mouse GAPDH ELISA Kit;Mouse peptidyl-cysteine S-nitrosylase GAPDH ELISA Kit;Mouse Gapd ELISA Kit;Mouse glyceraldehyde-3-phosphate dehydrogenase ELISA Kit;
Catalog
E053937
species
Mouse
GeneGapdh
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GAPDH. No significant cross-reactivity or interference between Mouse GAPDH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse GAPDH and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseMouse Gapdh ELISA Kit allows for the in vitro quantitative determination of Gapdh , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GAPDH in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAPDH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GAPDH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAPDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GAPDH. You can calculate the concentration of Mouse GAPDH in the samples by comparing the OD of the samples to the standard curve.
Mouse Glyceraldehyde-3-phosphate dehydrogenase Protein information
Uniprot ID G3P_MOUSE
Uniprot AC Q569X5; Q5U410;
UniGene Mm.304088; Mm.309092; Mm.317779; Mm.343110; Mm.392463; Mm.458138; Mm.458416;
GeneID 14433
KEGG mmu:14433;
Mouse Glyceraldehyde-3-phosphate dehydrogenase Protein SEQUENCE
SEQUENCE 333 AA; 35810 MW; F25131EFFA9F2BD6 CRC64;
MVKVGVNGFG RIGRLVTRAA ICSGKVEIVA INDPFIDLNY MVYMFQYDST
HGKFNGTVKA ENGKLVINGK PITIFQERDP TNIKWGEAGA EYVVESTGVF
TTMEKAGAHL KGGAKRVIIS APSADAPMFV MGVNHEKYDN SLKIVSNASC
TTNCLAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA
AQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTPNVSV VDLTCRLEKP
AKYDDIKKVV KQASEGPLKG ILGYTEDQVV SCDFNSNSHS STFDAGAGIA
LNDNFVKLIS WYDNEYGYSN RVVDLMAYMA SKE
UCSC uc007igj.1; mouse.;

Category

Mouse Gapdh ELISA Kit

Mouse GAPDH ELISA Kit;Mouse peptidyl-cysteine S-nitrosylase GAPDH ELISA Kit;Mouse Gapd ELISA Kit;Mouse glyceraldehyde-3-phosphate dehydrogenase ELISA Kit;