Mouse Gdf15 ELISA Kit

Alternative name
Mouse GDF-15 ELISA Kit;Mouse SBF ELISA Kit;Mouse MIC-1 ELISA Kit;Mouse NAG-1 ELISA Kit;Mouse growth differentiation factor 15 ELISA Kit;Mouse growth/differentiation factor 15 ELISA Kit;Mouse macrophage inhibiting cytokine-1 ELISA Kit;
Catalog
E054030
species
Mouse
GeneGdf15
SpecificityNatural and recombinant mouse GDF-15
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Gdf15 ELISA Kit allows for the in vitro quantitative determination of Gdf15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GDF-15 has been precoated onto 96-well plates. Standards(NSO, S189-A303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GDF-15 amount of sample captured in plate. Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
Mouse Growth/differentiation factor 15 Protein information
Uniprot ID GDF15_MOUSE
Uniprot AC Q9Z0J7; Q6NX63;
UniGene Mm.31325;
GeneID 23886
KEGG mmu:23886;
Mouse Growth/differentiation factor 15 Protein SEQUENCE
SEQUENCE 303 AA; 33225 MW; CD021EBEF598D3D0 CRC64;
MAPPALQAQP PGGSQLRFLL FLLLLLLLLS WPSQGDALAM PEQRPSGPES
QLNADELRGR FQDLLSRLHA NQSREDSNSE PSPDPAVRIL SPEVRLGSHG
QLLLRVNRAS LSQGLPEAYR VHRALLLLTP TARPWDITRP LKRALSLRGP
RAPALRLRLT PPPDLAMLPS GGTQLELRLR VAAGRGRRSA HAHPRDSCPL
GPGRCCHLET VQATLEDLGW SDWVLSPRQL QLSMCVGECP HLYRSANTHA
QIKARLHGLQ PDKVPAPCCV PSSYTPVVLM HRTDSGVSLQ TYDDLVARGC
HCA
UCSC uc009mba.2; mouse.;

Category

Mouse Gdf15 ELISA Kit

Mouse GDF-15 ELISA Kit;Mouse SBF ELISA Kit;Mouse MIC-1 ELISA Kit;Mouse NAG-1 ELISA Kit;Mouse growth differentiation factor 15 ELISA Kit;Mouse growth/differentiation factor 15 ELISA Kit;Mouse macrophage inhibiting cytokine-1 ELISA Kit;