SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse GLRX5. No significant cross-reactivity or interference between mouse GLRX5 and analogues was observed.
SensitivityThe minimum detectable dose of mouse GLRX5 is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseMouse Glrx5 ELISA Kit allows for the in vitro quantitative determination of Glrx5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLRX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLRX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLRX5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLRX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Glutaredoxin-related protein 5, mitochondrial Protein information
Uniprot ID
GLRX5_MOUSE
Uniprot AC
Q80Y14; Q3YML1; Q9D6E9;
UniGene
Mm.29128; Mm.490286;
GeneID
73046
KEGG
mmu:73046;
Mouse Glutaredoxin-related protein 5, mitochondrial Protein SEQUENCE