Mouse Gpc2 ELISA Kit

Alternative name
Mouse 2410016G05Rik ELISA Kit;Mouse glypican 2 (cerebroglycan) ELISA Kit;Mouse glypican-2 ELISA Kit;
Catalog
E054692
species
Mouse
GeneGpc2
SpecificityThis assay has high sensitivity and excellent specificity for detection of GPC2. No significant cross-reactivity or interference between GPC2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPC2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Gpc2 ELISA Kit allows for the in vitro quantitative determination of Gpc2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: GPC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC2 antibody and an GPC2-HRP conjugate. The assay sample and buffer are incubated together with GPC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC2 concentration since GPC2 from samples and GPC2-HRP conjugate compete for the anti-GPC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC2 from the sample, fewer sites are left to bind GPC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC2 concentration in each sample is interpolated from this standard curve.
Mouse Secreted glypican-2 Protein information
Uniprot ID GPC2_MOUSE
Uniprot AC Q8BKV1;
UniGene Mm.77066;
GeneID 71951
KEGG mmu:71951;
Mouse Secreted glypican-2 Protein SEQUENCE
SEQUENCE 579 AA; 63344 MW; BB2A604283F17CB0 CRC64;
MSALRPLLLL LLHLCPGLGP GHGSEAKVVR SCAETRQVLG ARGYSLNLIP
PSLISGEHLQ VCPQEYTCCS SETEQKLIRD AEVTFRGLVE DSGSFLIHTL
AARHRKFNEF FREMLSISQH SLAQLFSHSY GRLYSQHAVI FNSLFSGLRD
YYEKSGEGLD DTLADFWAQL LERAFPLLHP QYSFPPDFLL CLTRLTSTAD
GSLQPFGDSP RRLRLQISRA LVAARALVQG LETGRNVVSE ALKVPVLEGC
RQALMRLIGC PLCRGVPSLM PCRGFCLNVA HGCLSSRGLE PEWGGYLDGL
LLLAEKLQGP FSFELAAESI GVKISEGLMH LQENSVKVSA KVFQECGTPH
PVQSRSRRAP APREEASRSW RASAEEERPT TAAGTNLHRL VWELRERLSR
VRGFWAGLPV TVCGDSRMAA DLSQETAPCW TGVGRGRYMS PVVVGSLNEQ
LHNPELDTSS PDVPTRRRRL HLRAATARMK AAALGQDLDM HDADEDASGS
GGGQQYADDW KAGAVPVVPP ARPPRPPRPP RRDGLGVRGG SGSARYNQGR
SRNLGSSVGL HTPLVLLLLP SALTLLVLR
UCSC uc009afj.1; mouse.;

Category

Mouse Gpc2 ELISA Kit

Mouse 2410016G05Rik ELISA Kit;Mouse glypican 2 (cerebroglycan) ELISA Kit;Mouse glypican-2 ELISA Kit;