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Alternative name
Human secretin ELISA Kit;Human prepro-secretin ELISA Kit;
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Catalog
E034463
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species
Human
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GeneSCT
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretin (SCT).
No significant cross-reactivity or interference between Secretin (SCT) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 7.36pg/mL.
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Intended UseHuman SCT ELISA Kit allows for the in vitro quantitative determination of SCT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretin (SCT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretin (SCT) and unlabeled Secretin (SCT) (Standards or samples) with the pre-coated antibody specific to Secretin (SCT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretin (SCT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretin (SCT) in the sample.
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Human Secretin Protein information
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Uniprot ID
SECR_HUMAN
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Uniprot AC
P09683;
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UniGene
Hs.632324;
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GeneID
6343
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KEGG
hsa:6343;
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Human Secretin Protein SEQUENCE
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SEQUENCE 121 AA; 13016 MW; 44BDB4EFC0E161CF CRC64;
MAPRPLLLLL LLLGGSAARP APPRARRHSD GTFTSELSRL REGARLQRLL
QGLVGKRSEQ
DAENSMAWTR LSAGLLCPSG SNMPILQAWM PLDGTWSPWL
PPGPMVSEPA GAAAEGTLRP
R
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UCSC
uc001lqo.2; human.;
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