Mouse Hnf1a ELISA Kit

Alternative name
Mouse HNF-1-alpha ELISA Kit;Mouse HNF-1A ELISA Kit;Mouse liver-specific transcription factor LF-B1 ELISA Kit;Mouse LFB1 ELISA Kit;Mouse transcription factor 1 ELISA Kit;Mouse Tcf-1 ELISA Kit;Mouse Hnf-1 ELISA Kit;Mouse Tcf1 ELISA Kit;Mouse AI323641 ELISA Kit;Mouse HNF1 ELISA Kit;Mouse HNF1-alpha ELISA Kit;Mouse HNF1[a] ELISA Kit;Mouse Hnf1alpha ELISA Kit;Mouse HNF1 homeobox A ELISA Kit;Mouse hepatocyte nuclear factor 1-alpha ELISA Kit;Mouse hepatocyte nuclear factor 1 ELISA Kit;
Catalog
E055586
species
Mouse
GeneHnf1a
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 4.69pg/ml
Intended UseMouse Hnf1a ELISA Kit allows for the in vitro quantitative determination of Hnf1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse HNF1a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse HNF1a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse HNF1a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse HNF1a can be calculated.
Mouse Hepatocyte nuclear factor 1-alpha Protein information
Uniprot ID HNF1A_MOUSE
Uniprot AC P22361; E9QP86;
UniGene Mm.332607;
GeneID 21405
KEGG mmu:21405;
Mouse Hepatocyte nuclear factor 1-alpha Protein SEQUENCE
SEQUENCE 628 AA; 67253 MW; 737920D47BA1F081 CRC64;
MVSKLSQLQT ELLAALLESG LSKEALIQAL GEPGPYLMVG EGPLDKGESC
GGSRGDLTEL PNGLGETRGS EDDTDDDGED FAPPILKELE NLSPEEAAHQ
KAVVESLLQE DPWRVAKMVK SYLQQHNIPQ REVVDTTGLN QSHLSQHLNK
GTPMKTQKRA ALYTWYVRKQ REVAQQFTHA GQGGLIEEPT GDELPTKKGR
RNRFKWGPAS QQILFQAYER QKNPSKEERE TLVEECNRAE CIQRGVSPSQ
AQGLGSNLVT EVRVYNWFAN RRKEEAFRHK LAMDTYNGPP PGPGPGPALP
AHSSPGLPTT TLSPSKVHGV RYGQSATSEA AEVPSSSGGP LVTVSAALHQ
VSPTGLEPSS LLSTEAKLVS ATGGPLPPVS TLTALHSLEQ TSPGLNQQPQ
NLIMASLPGV MTIGPGEPAS LGPTFTNTGA STLVIGLAST QAQSVPVINS
MGSSLTTLQP VQFSQPLHPS YQQPLMPPVQ SHVAQSPFMA TMAQLQSPHA
LYSHKPEVAQ YTHTSLLPQT MLITDTNLST LASLTPTKQV FTSDTEASSE
PGLHEPPSPA TTIHIPSQDP SNIQHLQPAH RLSTSPTVSS SSLVLYQSSD
SNGHSHLLPS NHSVIETFIS TQMASSSQ
UCSC uc008zcz.2; mouse.;

Category

Mouse Hnf1a ELISA Kit