Mouse Hnf1b ELISA Kit

Alternative name
Mouse HNF-1-beta ELISA Kit;Mouse HNF-1B ELISA Kit;Mouse homeoprotein LFB3 ELISA Kit;Mouse transcription factor 2 ELISA Kit;Mouse Tcf-2 ELISA Kit;Mouse Tcf2 ELISA Kit;Mouse HNF-1Beta ELISA Kit;Mouse Hnf1beta ELISA Kit;Mouse LFB3 ELISA Kit;Mouse vHNF1 ELISA Kit;Mouse HNF1 homeobox B ELISA Kit;Mouse hepatocyte nuclear factor 1-beta ELISA Kit;
Catalog
E055589
species
Mouse
GeneHnf1b
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 4.69pg/ml
Intended UseMouse Hnf1b ELISA Kit allows for the in vitro quantitative determination of Hnf1b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse HNF1b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse HNF1b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse HNF1b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse HNF1b can be calculated.
Mouse Hepatocyte nuclear factor 1-beta Protein information
Uniprot ID HNF1B_MOUSE
Uniprot AC P27889; Q5NC37; Q8R162; Q9CS26; Q9R1W1; Q9R1W2; Q9WTL5; Q9WTL6;
UniGene Mm.7226;
GeneID 21410
KEGG mmu:21410;
Mouse Hepatocyte nuclear factor 1-beta Protein SEQUENCE
SEQUENCE 558 AA; 61588 MW; D4F2BC92B64374A6 CRC64;
MVSKLTSLQQ ELLSALLSSG VTKEVLIQAL EELLPSPNFG VKLETLPLSP
GSGADLDTKP VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT
EEAAEQRAEV DRMLSEDPWR AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL
SQHLNKGTPM KTQKRAALYT WYVRKQREIL RQFNQTVQSS GNMTDKSSQD
QLLFLFPEFS QQNQGPGQSE DTCSEPTNKK MRRNRFKWGP ASQQILYQAY
DRQKNPSKEE REALVEECNR AECLQRGVSP SKAHGLGSNL VTEVRVYNWF
ANRRKEEAFR QKLAMDAYSS NQTHNLNPLL THGSPHHQPS SSPPNKMSGV
RYNQPGNNEV TSSSTISHHG NSAMVTSQSV LQQVSPASLD PGHSLLSPDS
KMQITVSGGG LPPVSTLTNI HSLSHHNPQQ SQNLIMTPLS GVMAIAQSLN
TSQAQGVPVI NSVASSLAAL QPVQFSQQLH SPHQQPLMQQ SPGSHMAQQP
FMAAVTQLQN SHMYAHKQEP PQYSHTSRFP SAMVVTDTSS INTLTSMSSS
KQCPLQAW
UCSC uc007kpy.2; mouse. [P27889-2];

Category

Mouse Hnf1b ELISA Kit