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Mouse Hmgb4 ELISA Kit

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  • Alternative name

    Mouse 1700001F22Rik ELISA Kit;Mouse AI326135 ELISA Kit;Mouse high-mobility group box 4 ELISA Kit;Mouse high mobility group protein B4 ELISA Kit;
  • Catalog
    E055760
  • species
    Mouse
  • GeneHmgb4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<46.9pg/ml
  • Intended UseMouse Hmgb4 ELISA Kit allows for the in vitro quantitative determination of Hmgb4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Hmgb4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Hmgb4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Hmgb4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Hmgb4 can be calculated.
  • Mouse High mobility group protein B4 Protein information
  • Uniprot ID HMGB4_MOUSE
  • Uniprot AC Q6P8W9; Q9DAR4;
  • UniGene Mm.331893;
  • GeneID 69317
  • KEGG mmu:69317;
  • Mouse High mobility group protein B4 Protein SEQUENCE

  • SEQUENCE 181 AA; 21604 MW; A41FDE8F08413DBA CRC64;

    MGEKDQLRPK VNVSSYIHFM LNFRNKFKEQ QPNTYLGFKE FSRKCSEKWR

    SISKHEKAKY EALAELDKAR YQQEMMNYIG KRRKRRKRDP KAPRKPPSSF

    LLFSRDHYAM LKQENPDWTV VQVAKAAGKM WSTTDEAEKK PYEQKAALMR

    AKYFEEQEAY RNQCQGRKGN FLESAKTSLK Q

  • UCSC uc008uva.1; mouse.;



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