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Mouse Il1b ELISA Kit

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  • Alternative name

    Mouse IL-1 beta ELISA Kit;Mouse IL-1beta ELISA Kit;Mouse Il-1b ELISA Kit;Mouse interleukin 1 beta ELISA Kit;Mouse interleukin-1 beta ELISA Kit;
  • Catalog
    E056263
  • species
    Mouse
  • GeneIl1b
  • SpecificityNatural and recombinant mouse IL-1beta
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseMouse Il1b ELISA Kit allows for the in vitro quantitative determination of Il1b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: mouse IL-1beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1beta has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1beta amount of sample captured in plate. Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting inderepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore,Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotypemodulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may beexplained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
  • Mouse Interleukin-1 beta Protein information
  • Uniprot ID IL1B_MOUSE
  • Uniprot AC P10749; Q2M4J6;
  • UniGene Mm.222830;
  • GeneID 16176
  • KEGG mmu:16176;
  • Mouse Interleukin-1 beta Protein SEQUENCE

  • SEQUENCE 269 AA; 30931 MW; 734FA17B02ED87EE CRC64;

    MATVPELNCE MPPFDSDEND LFFEVDGPQK MKGCFQTFDL GCPDESIQLQ

    ISQQHINKSF RQAVSLIVAV EKLWQLPVSF PWTFQDEDMS TFFSFIFEEE

    PILCDSWDDD DNLLVCDVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG

    QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE

    SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF

    LGNNSGQDII DFTMESVSS

  • UCSC uc008mht.1; mouse.;



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