Mouse Il23a ELISA Kit

Alternative name
Mouse IL-23 subunit alpha ELISA Kit;Mouse IL-23-A ELISA Kit;Mouse interleukin-23 subunit p19 ELISA Kit;Mouse IL-23p19 ELISA Kit;Mouse IL-23 ELISA Kit;Mouse p19 ELISA Kit;Mouse interleukin 23, alpha subunit p19 ELISA Kit;Mouse interleukin-23 subunit alpha ELISA Kit;
Catalog
E056346
species
Mouse
GeneIl23a
SpecificityNatural and recombinant mouse IL-23
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseMouse Il23a ELISA Kit allows for the in vitro quantitative determination of Il23a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IL-23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-23 has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(L20-A196)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-23 amount of sample captured in plate. Background: Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. The International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).
Mouse Interleukin-23 subunit alpha Protein information
Uniprot ID IL23A_MOUSE
Uniprot AC Q9EQ14;
UniGene Mm.125482;
GeneID 83430
KEGG mmu:83430;
Mouse Interleukin-23 subunit alpha Protein SEQUENCE
SEQUENCE 196 AA; 22071 MW; DAF4A318A2DD3B7C CRC64;
MLDCRAVIML WLLPWVTQGL AVPRSSSPDW AQCQQLSRNL CMLAWNAHAP
AGHMNLLREE EDEETKNNVP RIQCEDGCDP QGLKDNSQFC LQRIRQGLAF
YKHLLDSDIF KGEPALLPDS PMEQLHTSLL GLSQLLQPED HPRETQQMPS
LSSSQQWQRP LLRSKILRSL QAFLAIAARV FAHGAATLTE PLVPTA
UCSC uc007hmc.1; mouse.;

Category

Mouse Il23a ELISA Kit

Mouse IL-23 subunit alpha ELISA Kit;Mouse IL-23-A ELISA Kit;Mouse interleukin-23 subunit p19 ELISA Kit;Mouse IL-23p19 ELISA Kit;Mouse IL-23 ELISA Kit;Mouse p19 ELISA Kit;Mouse interleukin 23, alpha subunit p19 ELISA Kit;Mouse interleukin-23 subunit alpha ELISA Kit;