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Alternative name
FG-GAP repeat protein ELISA KIT; FG-GAP repeat protein
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Catalog
E056853
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species
Mouse
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GeneITG/CD11b
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse ITG/CD11b ELISA Kit allows for the in vitro quantitative determination of ITG/CD11b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalFor Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Uses: This TGAM /CD11B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Moue TGAM /CD11B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: TGAM /CD11B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGAM /CD11B antibody and an TGAM /CD11B-HRP conjugate. The assay sample and buffer are incubated together with TGAM /CD11B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGAM /CD11B concentration since TGAM /CD11B from samples and TGAM /CD11B-HRP conjugate compete for the anti-TGAM /CD11B antibody binding site. Since the number of sites is limited, as more sites are occupied by TGAM /CD11B from the sample, fewer sites are left to bind TGAM /CD11B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGAM /CD11B concentration in each sample is interpolated from this standard curve.
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