Mouse Klf10 ELISA Kit

Alternative name
Mouse GDNF-inducible factor ELISA Kit;Mouse transcription factor GIF ELISA Kit;Mouse mGIF ELISA Kit;Mouse transforming growth factor-beta-inducible early growth response protein 1 ELISA Kit;Mouse TGFB-inducible early growth response protein 1 ELISA Kit;Mouse TIEG-1 ELISA Kit;Mouse Gdnfif ELISA Kit;Mouse Tieg ELISA Kit;Mouse Tieg1 ELISA Kit;Mouse AI115143 ELISA Kit;Mouse EGR[a] ELISA Kit;Mouse Egral ELISA Kit;Mouse Kruppel-like factor 10 ELISA Kit;Mouse Krueppel-like factor 10 ELISA Kit;Mouse TGFB inducible early growth response 1 ELISA Kit;
Catalog
E058138
species
Mouse
GeneKlf10
SpecificityThis assay recognizes recombinant and natural mouse Krueppel-like factor 10. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Klf10 ELISA Kit allows for the in vitro quantitative determination of Klf10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Krueppel-like factor 10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Krueppel-like factor 10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Krueppel-like factor 10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Krueppel-like factor 10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Krueppel-like factor 10 Protein information
Uniprot ID KLF10_MOUSE
Uniprot AC O89091;
UniGene Mm.4292;
GeneID 21847
KEGG mmu:21847;
Mouse Krueppel-like factor 10 Protein SEQUENCE
SEQUENCE 479 AA; 51756 MW; 99A5969665962963 CRC64;
MLNFGASLQQ ASEGKMELIS EKPREGMHPW DKAEQSDFEA VEALMSMSCD
WKSHFKKYLE NRPVTPVSDT SEDDSLLPGT PDLQTVPAFC LTPPYSPSDF
EPSQGSNLTA SAPSTGHFKS FSDAAKPPGA TPFKEEEKNP LAAPPLPKAQ
ATSVIRHTAD AQLCNHQSCP VKAASILNYQ DNSFRRRTHG NVEATRKNIP
CAAVSPNRSK PEPSTVSDGD EKAGAALYDF AVPSSETVIC RSQPAPSSPV
QKSVLVSSPT VSTGGVPPLP VICQMVPLPA NNSLVSTVVP STPPSQPPAV
CSPVLFMGTQ VPEGTVVFVV PQPVVQSPRP PVVSPSGTRL SPIAPAPGFS
PSAARVTPQI DSSRVRSHIC SHPGCGKTYF KSSHLKAHVR THTGEKPFSC
SWKGCERRFA RSDELSRHRR THTGEKKFAC PMCDRRFMRS DHLTKHARRH
LSAKKLPNWQ MEVSKLNDIA LPPTPASAQ
UCSC uc007vnr.2; mouse.;

Category

Mouse Klf10 ELISA Kit