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Mouse Tfpi ELISA Kit

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  • Alternative name

    Mouse TFPI ELISA Kit;Mouse extrinsic pathway inhibitor ELISA Kit;Mouse EPI ELISA Kit;Mouse lipoprotein-associated coagulation inhibitor ELISA Kit;Mouse LACI ELISA Kit;Mouse A630013F22Rik ELISA Kit;Mouse AW552122 ELISA Kit;Mouse tissue factor pathway inhibitor ELISA Kit;
  • Catalog
    E058227
  • species
    Mouse
  • GeneTfpi
  • SpecificityThe Mouse LACI ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse LACI proteins within the range of 156 pg/ml - 10000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseMouse Tfpi ELISA Kit allows for the in vitro quantitative determination of Tfpi , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Mouse LACI ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse LACI in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse LACI coated on a 96-well plate. Standards and samples are pipetted into the wells and LACI present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse LACI antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LACI bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.
  • Mouse Tissue factor pathway inhibitor Protein information
  • Uniprot ID TFPI1_MOUSE
  • Uniprot AC O54819; Q9Z2U8;
  • UniGene Mm.124316; Mm.447013;
  • GeneID 21788
  • KEGG mmu:21788;
  • Mouse Tissue factor pathway inhibitor Protein SEQUENCE

  • SEQUENCE 306 AA; 34987 MW; D3EA3297E4B6A359 CRC64;

    MTYKMKKEYA FWATVCLLLS LVPEFLNALS EEADDTDSEL GSMKPLHTFC

    AMKADDGPCK AMIRSYFFNM YTHQCEEFIY GGCEGNENRF DTLEECKKTC

    IPGYEKTAVK AASGAERPDF CFLEEDPGLC RGYMKRYLYN NQTKQCERFV

    YGGCLGNRNN FETLDECKKI CENPVHSPSP VNEVQMSDYV TDGNTVTDRS

    TVNNIVVPQS PKVPRRRDYR GRPWCLQPAD SGLCKASERR FYYNSATGKC

    HRFNYTGCGG NNNNFTTRRR CLRSCKTGLI KNKSKGVVKI QRRKAPFVKV

    VYESIN

  • UCSC uc008kim.1; mouse. [O54819-2];



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