SpecificityThis assay recognizes recombinant and natural mouse LPS-induced TNF-alpha factor homolog. No significant cross-reactivity or interference was observed.
Intended UseMouse Litaf ELISA Kit allows for the in vitro quantitative determination of Litaf , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LPS-induced TNF-alpha factor homolog. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LPS-induced TNF-alpha factor homolog and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LPS-induced TNF-alpha factor homolog, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LPS-induced TNF-alpha factor homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog Protein information
Uniprot ID
LITAF_MOUSE
Uniprot AC
Q9JLJ0; Q9EQI0;
UniGene
Mm.294753;
GeneID
56722
KEGG
mmu:56722;
Mouse Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog Protein SEQUENCE