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Mouse MIP1b ELISA Kit

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  • Alternative name

    C-C motif chemokine 4 ELISA KIT; Macrophage inflammatory protein 1-beta ELISA KIT; MIP-1-beta ELISA KIT; Small-inducible cytokine A4CCL4 ELISA KIT; MIP1B ELISA KIT; SCYA4 ELISA KIT; MIP-1-beta ELISA KIT
  • Catalog
    E059268
  • species
    Mouse
  • GeneMIP1b
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Beta (MIP1b). No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Beta (MIP1b) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 5.2pg/mL.
  • Intended UseMouse MIP1b ELISA Kit allows for the in vitro quantitative determination of MIP1b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta (MIP1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Beta (MIP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Beta (MIP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Beta (MIP1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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