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Alternative name
N-lysine methyltransferase SMYD2 ELISA KIT; HSKM-B ELISA KIT; Histone methyltransferase SMYD2 (EC:2.1.1.43) ELISA KIT; Lysine N-methyltransferase 3C ELISA KIT; SET and MYND domain-containing protein 2SMYD2 ELISA KIT; KMT3C ELISA KIT
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Catalog
E060999
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species
Mouse
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GeneSMYD2
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SpecificityThis assay has high sensitivity and excellent specificity for detection of SMYD2. No significant cross-reactivity or interference between SMYD2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SMYD2 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse SMYD2 ELISA Kit allows for the in vitro quantitative determination of SMYD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyEpigenetics and Nuclear Signaling
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Product Description
specificalIntended Uses: This SMYD2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SMYD2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||SMYD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SMYD2 antibody and an SMYD2-HRP conjugate. The assay sample and buffer are incubated together with SMYD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SMYD2 concentration since SMYD2 from samples and SMYD2-HRP conjugate compete for the anti-SMYD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SMYD2 from the sample, fewer sites are left to bind SMYD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SMYD2 concentration in each sample is interpolated from this standard curve.
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