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Mouse Pon1 ELISA Kit

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  • Alternative name

    Mouse PON 1 ELISA Kit;Mouse aromatic esterase 1 ELISA Kit;Mouse A-esterase 1 ELISA Kit;Mouse serum aryldialkylphosphatase 1 ELISA Kit;Mouse Pon ELISA Kit;Mouse paraoxonase 1 ELISA Kit;Mouse serum paraoxonase/arylesterase 1 ELISA Kit;
  • Catalog
    E062981
  • species
    Mouse
  • GenePon1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Paraoxonase 1 (PON1). No significant cross-reactivity or interference between Paraoxonase 1 (PON1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.57ng/mL.
  • Intended UseMouse Pon1 ELISA Kit allows for the in vitro quantitative determination of Pon1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraoxonase 1 (PON1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paraoxonase 1 (PON1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraoxonase 1 (PON1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paraoxonase 1 (PON1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Serum paraoxonase/arylesterase 1 Protein information
  • Uniprot ID PON1_MOUSE
  • Uniprot AC P52430; Q91X30;
  • UniGene Mm.237657;
  • GeneID 18979
  • KEGG mmu:18979;
  • Mouse Serum paraoxonase/arylesterase 1 Protein SEQUENCE

  • SEQUENCE 355 AA; 39565 MW; FFA0A71445BB80B4 CRC64;

    MAKLLALTLV GLVLALYKNH RSSYQTRLNA FREVTPVELP NCNLVKGIET

    GAEDLEILPN GLTFFSTGLK YPGIKSFDPS KPGKILLMDL NKKEPAVSEL

    EIIGNTLDIS SFNPHGISTF TDEDNTVYLL VVNHPDSSST VEVFKFQEEE

    RSLLHLKTIT HELLPSINDI AAIGPESFYA TNDHYFADPY LRSWEMYLGL

    SWSNVVYYSP DKVQVVAEGF DFANGIGISL DGKYVYIAEL LAHKIHVYEK

    HANWTLTPLK VLNFDTLVDN ISVDPVTGDL WVGCHPNGMR IFFYDAENPP

    GSEVLRIQNI LSEDPKITVV YAENGTVLQG TTVASVYKGK LLIGTVFHKA

    LYCDL

  • UCSC uc009awd.2; mouse.;



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