Mouse Pkn1 ELISA Kit

Alternative name
Mouse protein kinase C-like 1 ELISA Kit;Mouse protein kinase C-like PKN ELISA Kit;Mouse protein-kinase C-related kinase 1 ELISA Kit;Mouse serine-threonine protein kinase N ELISA Kit;Mouse Pkn ELISA Kit;Mouse PRK1 ELISA Kit;Mouse Prkcl1 ELISA Kit;Mouse DBK ELISA Kit;Mouse F730027O18Rik ELISA Kit;Mouse PAK1 ELISA Kit;Mouse Stk3 ELISA Kit;Mouse protein kinase N1 ELISA Kit;Mouse serine/threonine-protein kinase N1 ELISA Kit;Mouse serine/threonine kinase 3 ELISA Kit;
Catalog
E063839
species
Mouse
GenePkn1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PKN1. No significant cross-reactivity or interference between Mouse PKN1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PKN1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Pkn1 ELISA Kit allows for the in vitro quantitative determination of Pkn1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PKN1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PKN1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PKN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PKN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PKN1. You can calculate the concentration of Mouse PKN1 in the samples by comparing the OD of the samples to the standard curve.
Mouse Serine/threonine-protein kinase N1 Protein information
Uniprot ID PKN1_MOUSE
Uniprot AC P70268; Q3UEA6; Q7TST2; Q8BTL8;
UniGene Mm.213000; Mm.440344;
GeneID 320795
KEGG mmu:320795;
Mouse Serine/threonine-protein kinase N1 Protein SEQUENCE
SEQUENCE 946 AA; 104411 MW; FCB4E61AD27C1271 CRC64;
MAGDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLKREIRKEL
KLKEGAENLR RATTDLGRSL APVELLLRGS ARRLDLLHQQ LQELHAHVVL
PDPAAGSDAT QSLAEGSPIC SSTNLSRVAG LEKQLAIELK VKQGAENMIQ
TYSNGSSKDR KLLLTAQQML QDSKTKIDII RMQLRRALQA LQAGELESQA
APDEAQGDPE LGAVELRIEE LRHHFRVEHA VAEGAKNVLR LLSGAKAPDR
KAVSEAQEKL TESNQKLGLL RESLERRLGE LPADHPKGRL LREELTAASS
SAFSAILPGP FPATHYSTLS KPAPLTGTLE VRVVGCKNLP ETIPWSPPPS
VGASGTPESR TPFLSRPARG LYSRSGSLSG RSSLRGEAEN ATEVSTVLKL
DNTVVGQTAW KPCGPNAWDQ SFTLELERAR ELELAVFWRD QRGLCALKFL
KLEDFLDNER HEVQLDMEPQ GCLVAEVTFR NPIIERIPRL QRQKKIFSKQ
QGKAFQRARQ MNIDVATWVR LLRRLIPSAV ATGTFSPNAS PGAEIRHTGD
ISMEKLNLGA DSDSSSQKSP PGLPSTSCSL SSPTHESTTS PELPSETQET
PGPGLCSPLR KSPLTLEDFK FLAVLGRGHF GKVLLSEFRS SGELFAIKAL
KKGDIVARDE VESLMCEKRI LAAVTRAGHP FLVNLFGCFQ TPEHVCFVME
YSAGGDLMLH IHSDVFSEPR AVFYSACVVL GLQFLHEHKI VYRDLKLDNL
LLDTEGYVKI ADFGLCKEGM GYGDRTSTFC GTPEFLAPEV LTDTSYTRAV
DWWGLGVLLY EMLVGESPFP GDDEEEVFDS IVNDEVRYPR FLSAEAIGIM
RRLLRRNPER RLGSTERDAE DVKKQPFFRS LGWDVLLARR LPPPFVPTLS
GRTDVSNFDE EFTGEAPTLS PPRDARPLTA AEQAAFRDFD FVAGGY
UCSC uc009mkx.2; mouse. [P70268-1];

Category

Mouse Pkn1 ELISA Kit