Mouse Acpp ELISA Kit

Alternative name
Mouse 5'-nucleotidase ELISA Kit;Mouse 5'-NT ELISA Kit;Mouse ecto-5'-nucleotidase ELISA Kit;Mouse fluoride-resistant acid phosphatase ELISA Kit;Mouse FRAP ELISA Kit;Mouse thiamine monophosphatase ELISA Kit;Mouse TMPase ELISA Kit;Mouse A030005E02Rik ELISA Kit;Mouse Lap ELISA Kit;Mouse PAP ELISA Kit;Mouse Ppal ELISA Kit;Mouse acid phosphatase, prostate ELISA Kit;Mouse prostatic acid phosphatase ELISA Kit;Mouse lysosomal acid phosphatase ELISA Kit;
Catalog
E064941
species
Mouse
GeneAcpp
SpecificityThis assay recognizes recombinant and natural mouse Prostatic acid phosphatase. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Acpp ELISA Kit allows for the in vitro quantitative determination of Acpp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostatic acid phosphatase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Prostatic acid phosphatase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Prostatic acid phosphatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Prostatic acid phosphatase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Prostatic acid phosphatase Protein information
Uniprot ID PPAP_MOUSE
Uniprot AC Q8CE08; A4QPG2; B8JJZ5; B8JJZ6; Q8C682; Q9QXH7;
UniGene Mm.19941;
GeneID 56318
KEGG mmu:56318;
Mouse Prostatic acid phosphatase Protein SEQUENCE
SEQUENCE 381 AA; 43717 MW; C0077819D77CB251 CRC64;
MRAVPLPLSR TASLSLGFLL LLSLCLDPGQ AKELKFVTLV FRHGDRGPIE
TFPTDPITES SWPQGFGQLT QWGMEQHYEL GSYIRKRYGR FLNDTYKHDQ
IYIRSTDVDR TLMSAMTNLA ALFPPEGISI WNPRLLWQPI PVHTVSLSED
RLLYLPFRDC PRFEELKSET LESEEFLKRL HPYKSFLDTL SSLSGFDDQD
LFGIWSKVYD PLFCESVHNF TLPSWATEDA MIKLKELSEL SLLSLYGIHK
QKEKSRLQGG VLVNEILKNM KLATQPQKYK KLVMYSAHDT TVSGLQMALD
VYNGVLPPYA SCHMMELYHD KGGHFVEMYY RNETQNEPYP LTLPGCTHSC
PLEKFAELLD PVISQDWATE CMATSSHQGR N
UCSC uc009rhm.1; mouse. [Q8CE08-1];

Category

Mouse Acpp ELISA Kit