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Alternative name
Prostatic acid phosphatase ELISA KIT; 5'-nucleotidase (EC:3.1.3.5) ELISA KIT; 5'-NT ELISA KIT; Ecto-5'-nucleotidase ELISA KIT; Fluoride-resistant acid phosphatase ELISA KIT; FRAP ELISA KIT; Thiamine monophosphatase ELISA KIT; TMPaseAcpp ELISA KIT; 5'-NT ELISA KIT; FRAP ELISA KIT; TMPase ELISA KIT
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Catalog
E064945
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species
Mouse
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GenePAP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse PAP. No significant cross-reactivity or interference between mouse PAP and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse PAP is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
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Intended UseMouse PAP ELISA Kit allows for the in vitro quantitative determination of PAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrincple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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