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Catalog
E065664
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species
Mouse
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GenePDHa
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Alpha (PDHa).
No significant cross-reactivity or interference between Pyruvate Dehydrogenase Alpha (PDHa) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 34pg/mL.
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Intended UseMouse PDHa ELISA Kit allows for the in vitro quantitative determination of PDHa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Alpha (PDHa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Alpha (PDHa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Alpha (PDHa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Alpha (PDHa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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