Mouse Sesn3 ELISA Kit

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  • Alternative name

    Mouse 5630400E15Rik ELISA Kit;Mouse BC003348 ELISA Kit;Mouse SEST3 ELISA Kit;Mouse sestrin 3 ELISA Kit;Mouse sestrin-3 ELISA Kit;

  • Catalog
    E067557
  • species
    Mouse
  • GeneSesn3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse SESN3. No significant cross-reactivity or interference between Mouse SESN3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse SESN3 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Sesn3 ELISA Kit allows for the in vitro quantitative determination of Sesn3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SESN3 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SESN3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse SESN3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SESN3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SESN3. You can calculate the concentration of Mouse SESN3 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Sestrin-3 Protein information
  • Uniprot ID SESN3_MOUSE
  • Uniprot AC Q9CYP7; Q3U2A0;
  • UniGene Mm.325126; Mm.439776;
  • GeneID 75747
  • KEGG mmu:75747;
  • Mouse Sestrin-3 Protein SEQUENCE
  • SEQUENCE 492 AA; 57021 MW; 8E8AD9CB45656827 CRC64;

    MNRGGSSASA SANYLLCTNC RKVLRKDKRI RVSQPLTRGP SAFIPEKEVV

    QANTADERTN FLVEEYSTSG RLDNITQVMS LHTQYLESFL RSQFYMLRMD

    GPLPLPDRHY IAIMAAARHQ CSYLINMHVD EFLKTGGIAE WLNGLEYVPQ

    RLRNLNEINK LLAHRPWLIT KEHIQKLVKT GENNWSLPEL VHAVVLLAHY

    HALASFVFGS GINPERDPGI ANGFRLISVS SFCVCDLAND NSIENTSLAG

    SNFGIVDSLG ELEALMERMK RLQEDREDDE TTREEMTTRF EKEKKESLFV

    VPGETLHAFP HSDFEDDVIV TADVSRYIED PSFGYEDFAR RGEEHLPTFR

    AQDYTWENHG FSLVNRLYSD IGHLLDEKFR MVYNLTYNTM ATHEDVDTTT

    LRRALFNYVH CMFGIRYDDY DYGEVNQLLE RSLKVYIKTV TCYPERTTKR

    MYDSYWRQFT HSEKVHVNLL LMEARMQAEL LYALRAITRH LT

  • UCSC uc009oej.1; mouse.;



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