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Mouse Sirt1 ELISA Kit

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  • Alternative name

    Mouse regulatory protein SIR2 homolog 1 ELISA Kit;Mouse SIR2-like protein 1 ELISA Kit;Mouse Sir2alpha ELISA Kit;Mouse Sir2 ELISA Kit;Mouse mSIR2a ELISA Kit;Mouse 75SirT1 ELISA Kit;Mouse SIR2L1 ELISA Kit;Mouse AA673258 ELISA Kit;Mouse Sir2a ELISA Kit;Mouse sirtuin 1 ELISA Kit;Mouse NAD-dependent protein deacetylase sirtuin-1 ELISA Kit;Mouse sirtuin 1 ((silent mating type information regulation 2, homolog) 1 ELISA Kit;
  • Catalog
    E067840
  • species
    Mouse
  • GeneSirt1
  • SpecificityThis kit recognizes natural and recombinant Mouse SIRT1. No significant cross-reactivity or interference between Mouse SIRT1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Sirt1 ELISA Kit allows for the in vitro quantitative determination of Sirt1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse SIRT1 concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SIRT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SIRT1. You can calculate the concentration of SIRT1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse SirtT1 75 kDa fragment Protein information
  • Uniprot ID SIR1_MOUSE
  • Uniprot AC Q923E4; Q9QXG8;
  • UniGene Mm.351459;
  • GeneID 93759
  • KEGG mmu:93759;
  • Mouse SirtT1 75 kDa fragment Protein SEQUENCE

  • SEQUENCE 737 AA; 80372 MW; 7F15625E29433119 CRC64;

    MADEVALALQ AAGSPSAAAA MEAASQPADE PLRKRPRRDG PGLGRSPGEP

    SAAVAPAAAG CEAASAAAPA ALWREAAGAA ASAEREAPAT AVAGDGDNGS

    GLRREPRAAD DFDDDEGEEE DEAAAAAAAA AIGYRDNLLL TDGLLTNGFH

    SCESDDDDRT SHASSSDWTP RPRIGPYTFV QQHLMIGTDP RTILKDLLPE

    TIPPPELDDM TLWQIVINIL SEPPKRKKRK DINTIEDAVK LLQECKKIIV

    LTGAGVSVSC GIPDFRSRDG IYARLAVDFP DLPDPQAMFD IEYFRKDPRP

    FFKFAKEIYP GQFQPSLCHK FIALSDKEGK LLRNYTQNID TLEQVAGIQR

    ILQCHGSFAT ASCLICKYKV DCEAVRGDIF NQVVPRCPRC PADEPLAIMK

    PEIVFFGENL PEQFHRAMKY DKDEVDLLIV IGSSLKVRPV ALIPSSIPHE

    VPQILINREP LPHLHFDVEL LGDCDVIINE LCHRLGGEYA KLCCNPVKLS

    EITEKPPRPQ KELVHLSELP PTPLHISEDS SSPERTVPQD SSVIATLVDQ

    ATNNNVNDLE VSESSCVEEK PQEVQTSRNV ENINVENPDF KAVGSSTADK

    NERTSVAETV RKCWPNRLAK EQISKRLEGN QYLFVPPNRY IFHGAEVYSD

    SEDDVLSSSS CGSNSDSGTC QSPSLEEPLE DESEIEEFYN GLEDDTERPE

    CAGGSGFGAD GGDQEVVNEA IATRQELTDV NYPSDKS

  • UCSC uc007fke.2; mouse. [Q923E4-1];



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