Human CASP9 ELISA Kit

Alternative name
Human CASP-9 ELISA Kit;Human apoptotic protease MCH-6 ELISA Kit;Human Apoptotic protease-activating factor 3 ELISA Kit;Human APAF-3 ELISA Kit;Human ICE-like apoptotic protease 6 ELISA Kit;Human ICE-LAP6 ELISA Kit;Human MCH6 ELISA Kit;Human APAF3 ELISA Kit;Human PPP1R56 ELISA Kit;Human caspase 9 ELISA Kit;Human caspase-9 ELISA Kit;Human apoptotic protease activating factor 3 ELISA Kit;Human caspase 9, apoptosis-related cysteine peptidase ELISA Kit;Human protein phosphatase 1, regulatory subunit 56 ELISA Kit;
Catalog
E006897
species
Human
GeneCASP9
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CASP9. No significant cross-reactivity or interference between Human CASP9 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.195 ng/ml
Intended UseHuman CASP9 ELISA Kit allows for the in vitro quantitative determination of CASP9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Caspase-9 is an initiator caspase, encoded by the CASP9 gene. CASP9 orthologs have been identified in all mammals for which complete genome data are available. Unique orthologs are also present in lizards, lissamphibians, and teleosts. The aspartic acid specific protease caspase-9 has been linked to the mitochondrial death pathway. It is activated during programmed cell death (apoptosis). Induction of stress signalling pathways JNK/SAPK causes release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which in turn cleaves the pro-enzyme of caspase-9 into the active form. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casp-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Casp-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Casp-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Casp-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Caspase-9 subunit p10 Protein information
Uniprot ID CASP9_HUMAN
Uniprot AC Q9UEQ3; Q9UIJ8;
UniGene Hs.329502;
GeneID 842
KEGG hsa:842;
Human Caspase-9 subunit p10 Protein SEQUENCE
SEQUENCE 416 AA; 46281 MW; 78E0180DF2A3BDD2 CRC64;
MDEADRRLLR RCRLRLVEEL QVDQLWDALL SRELFRPHMI EDIQRAGSGS
RRDQARQLII DLETRGSQAL PLFISCLEDT GQDMLASFLR TNRQAAKLSK
PTLENLTPVV LRPEIRKPEV LRPETPRPVD IGSGGFGDVG ALESLRGNAD
LAYILSMEPC GHCLIINNVN FCRESGLRTR TGSNIDCEKL RRRFSSLHFM
VEVKGDLTAK KMVLALLELA QQDHGALDCC VVVILSHGCQ ASHLQFPGAV
YGTDGCPVSV EKIVNIFNGT SCPSLGGKPK LFFIQACGGE QKDHGFEVAS
TSPEDESPGS NPEPDATPFQ EGLRTFDQLD AISSLPTPSD IFVSYSTFPG
FVSWRDPKSG SWYVETLDDI FEQWAHSEDL QSLLLRVANA VSVKGIYKQM
PGCFNFLRKK LFFKTS
UCSC uc001awn.5; human. [P55211-1];

Category

Human CASP9 ELISA Kit