Mouse Tlr3 ELISA Kit

Alternative name
Mouse AI957183 ELISA Kit;Mouse toll-like receptor 3 ELISA Kit;
Catalog
E070350
species
Mouse
GeneTlr3
SpecificityThis assay has high sensitivity and excellent specificity for detection of TLR3. No significant cross-reactivity or interference between TLR3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TLR3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Tlr3 ELISA Kit allows for the in vitro quantitative determination of Tlr3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: TLR3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLR3 antibody and an TLR3-HRP conjugate. The assay sample and buffer are incubated together with TLR3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR3 concentration since TLR3 from samples and TLR3-HRP conjugate compete for the anti-TLR3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR3 from the sample, fewer sites are left to bind TLR3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR3 concentration in each sample is interpolated from this standard curve.
Mouse Toll-like receptor 3 Protein information
Uniprot ID TLR3_MOUSE
Uniprot AC Q99MB1; Q91ZM4;
UniGene Mm.33874;
GeneID 142980
KEGG mmu:142980;
Mouse Toll-like receptor 3 Protein SEQUENCE
SEQUENCE 905 AA; 103671 MW; 8EA6DBA9818E14B4 CRC64;
MKGCSSYLMY SFGGLLSLWI LLVSSTNQCT VRYNVADCSH LKLTHIPDDL
PSNITVLNLT HNQLRRLPPT NFTRYSQLAI LDAGFNSISK LEPELCQILP
LLKVLNLQHN ELSQISDQTF VFCTNLTELD LMSNSIHKIK SNPFKNQKNL
IKLDLSHNGL SSTKLGTGVQ LENLQELLLA KNKILALRSE ELEFLGNSSL
RKLDLSSNPL KEFSPGCFQT IGKLFALLLN NAQLNPHLTE KLCWELSNTS
IQNLSLANNQ LLATSESTFS GLKWTNLTQL DLSYNNLHDV GNGSFSYLPS
LRYLSLEYNN IQRLSPRSFY GLSNLRYLSL KRAFTKQSVS LASHPNIDDF
SFQWLKYLEY LNMDDNNIPS TKSNTFTGLV SLKYLSLSKT FTSLQTLTNE
TFVSLAHSPL LTLNLTKNHI SKIANGTFSW LGQLRILDLG LNEIEQKLSG
QEWRGLRNIF EIYLSYNKYL QLSTSSFALV PSLQRLMLRR VALKNVDISP
SPFRPLRNLT ILDLSNNNIA NINEDLLEGL ENLEILDFQH NNLARLWKRA
NPGGPVNFLK GLSHLHILNL ESNGLDEIPV GVFKNLFELK SINLGLNNLN
KLEPFIFDDQ TSLRSLNLQK NLITSVEKDV FGPPFQNLNS LDMRFNPFDC
TCESISWFVN WINQTHTNIS ELSTHYLCNT PHHYYGFPLK LFDTSSCKDS
APFELLFIIS TSMLLVFILV VLLIHIEGWR ISFYWNVSVH RILGFKEIDT
QAEQFEYTAY IIHAHKDRDW VWEHFSPMEE QDQSLKFCLE ERDFEAGVLG
LEAIVNSIKR SRKIIFVITH HLLKDPLCRR FKVHHAVQQA IEQNLDSIIL
IFLQNIPDYK LNHALCLRRG MFKSHCILNW PVQKERINAF HHKLQVALGS
RNSAH
UCSC uc009loy.1; mouse.;

Category

Mouse Tlr3 ELISA Kit

Mouse AI957183 ELISA Kit;Mouse toll-like receptor 3 ELISA Kit;