Human CTSF ELISA Kit

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  • Alternative name

    Human CATSF ELISA Kit;Human CLN13 ELISA Kit;Human cathepsin F ELISA Kit;

  • Catalog
    E007038
  • species
    Human
  • GeneCTSF
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<46.9pg/ml
  • Intended UseHuman CTSF ELISA Kit allows for the in vitro quantitative determination of CTSF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CTSF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CTSF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CTSF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CTSF can be calculated.
  • Human Cathepsin F Protein information
  • Uniprot ID CATF_HUMAN
  • Uniprot AC Q9UBX1; B2R964; O95240; Q9NSU4; Q9UKQ5;
  • UniGene Hs.11590;
  • GeneID 8722
  • KEGG hsa:8722;
  • Human Cathepsin F Protein SEQUENCE
  • SEQUENCE 484 AA; 53366 MW; 1D5D551B489D822B CRC64;

    MAPWLQLLSL LGLLPGAVAA PAQPRAASFQ AWGPPSPELL APTRFALEMF

    NRGRAAGTRA VLGLVRGRVR RAGQGSLYSL EATLEEPPCN DPMVCRLPVS

    KKTLLCSFQV LDELGRHVLL RKDCGPVDTK VPGAGEPKSA FTQGSAMISS

    LSQNHPDNRN ETFSSVISLL NEDPLSQDLP VKMASIFKNF VITYNRTYES

    KEEARWRLSV FVNNMVRAQK IQALDRGTAQ YGVTKFSDLT EEEFRTIYLN

    TLLRKEPGNK MKQAKSVGDL APPEWDWRSK GAVTKVKDQG MCGSCWAFSV

    TGNVEGQWFL NQGTLLSLSE QELLDCDKMD KACMGGLPSN AYSAIKNLGG

    LETEDDYSYQ GHMQSCNFSA EKAKVYINDS VELSQNEQKL AAWLAKRGPI

    SVAINAFGMQ FYRHGISRPL RPLCSPWLID HAVLLVGYGN RSDVPFWAIK

    NSWGTDWGEK GYYYLHRGSG ACGVNTMASS AVVD

  • UCSC uc001oip.4; human.;



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