Human CEBPA ELISA Kit

Alternative name
Human C/EBP alpha ELISA Kit;Human C/EBP-alpha ELISA Kit;Human CEBP ELISA Kit;Human CCAAT/enhancer binding protein alpha ELISA Kit;Human CCAAT/enhancer-binding protein alpha ELISA Kit;Human CCAAT/enhancer binding protein (C/EBP), alpha ELISA Kit;
Catalog
E007159
species
Human
GeneCEBPA
SpecificityThis assay has high sensitivity and excellent specificity for detection of CEBPa. No significant cross-reactivity or interference between CEBPa and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CEBPa is typically less than 0.055ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman CEBPA ELISA Kit allows for the in vitro quantitative determination of CEBPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CEBPa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CEBPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CEBPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CEBPa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human CCAAT/enhancer-binding protein alpha Protein information
Uniprot ID CEBPA_HUMAN
Uniprot AC P49715; A7LNP2; P78319; Q05CA4;
UniGene Hs.76171;
GeneID 1050
KEGG hsa:1050;
Human CCAAT/enhancer-binding protein alpha Protein SEQUENCE
SEQUENCE 358 AA; 37561 MW; 574C0A049E25BCAC CRC64;
MESADFYEAE PRPPMSSHLQ SPPHAPSSAA FGFPRGAGPA QPPAPPAAPE
PLGGICEHET SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAVGPTGG
GGGGDFDYPG APAGPGGAVM PGGAHGPPPG YGCAAAGYLD GRLEPLYERV
GAPALRPLVI KQEPREEDEA KQLALAGLFP YQPPPPPPPS HPHPHPPPAH
LAAPHLQFQI AHCGQTTMHL QPGHPTPPPT PVPSPHPAPA LGAAGLPGPG
SALKGLGAAH PDLRASGGSG AGKAKKSVDK NSNEYRVRRE RNNIAVRKSR
DKAKQRNVET QQKVLELTSD NDRLRKRVEQ LSRELDTLRG IFRQLPESSL
VKAMGNCA
UCSC uc002nun.4; human. [P49715-1];

Category

Human CEBPA ELISA Kit

Human C/EBP alpha ELISA Kit;Human C/EBP-alpha ELISA Kit;Human CEBP ELISA Kit;Human CCAAT/enhancer binding protein alpha ELISA Kit;Human CCAAT/enhancer-binding protein alpha ELISA Kit;Human CCAAT/enhancer binding protein (C/EBP), alpha ELISA Kit;